Separation of rare cell subpopulations with the aid of biotin-labelled ligands

Michael Steinitz; Alejandro Livoff; Sara Tamir; Talma Brenner

doi:10.1007/BF02987768

Separation of rare cell subpopulations with the aid of biotin-labelled ligands

Michael Steinitz^*
, Alejandro Livoff
, Sara Tamir
, Talma Brenner

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are In-st labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10^-4. The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).

Original language	English
Pages (from-to)	49-52
Number of pages	4
Journal	Medical Oncology and Tumor Pharmacotherapy
Volume	10
Issue number	1-2
DOIs	https://doi.org/10.1007/BF02987768
State	Published - Mar 1993

Keywords

Biotin
Human monoclonal antibodies
Rosette
Surface marker
lmmuno-selection

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF02987768

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abstract = "A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are In-st labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10-4. The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).",

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author = "Michael Steinitz and Alejandro Livoff and Sara Tamir and Talma Brenner",

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AU - Steinitz, Michael

AU - Livoff, Alejandro

AU - Tamir, Sara

AU - Brenner, Talma

PY - 1993/3

Y1 - 1993/3

N2 - A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are In-st labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10-4. The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).

AB - A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are In-st labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10-4. The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).

KW - Biotin

KW - Human monoclonal antibodies

KW - Rosette

KW - Surface marker

KW - lmmuno-selection

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JF - Medical Oncology and Tumor Pharmacotherapy

IS - 1-2

ER -

Separation of rare cell subpopulations with the aid of biotin-labelled ligands

Abstract

Keywords

UN SDGs

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