Separation of roles of Zip1 in meiosis revealed in heterozygous mutants of Saccharomyces cerevisiae

Michael Klutstein; Martin Xaver; Ronen Shemesh; Drora Zenvirth; Franz Klein; Giora Simchen

doi:10.1007/s00438-009-0477-z

Separation of roles of Zip1 in meiosis revealed in heterozygous mutants of Saccharomyces cerevisiae

Michael Klutstein^*, Martin Xaver, Ronen Shemesh, Drora Zenvirth, Franz Klein, Giora Simchen

^*Corresponding author for this work

Institute of Biomedical Research at the Faculty of Dental Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Synapsis of homologs during meiotic prophase I is associated with a protein complex built along the bivalents-the synaptonemal complex (SC). Mutations in the SC-component gene ZIP1 diminish SC formation, leading to reduced recombination levels and low spore viability. Here we show that in SK1 strains heterozygous for a deletion of ZIP1 in certain regions meiotic interference are impaired with no decrease in recombination levels. The extent of synapsis is over all reduced and NDJ levels of a large endogenous chromosome and of artificial chromosomes (YACs) rise to twice the level of wild type strains. A substantial proportion of mis-segregating YACs had undergone crossing over. This demonstrates that different functions of Zip1 display differential sensitivities to changes in expression levels.

Original language	English
Pages (from-to)	453-462
Number of pages	10
Journal	Molecular Genetics and Genomics
Volume	282
Issue number	5
DOIs	https://doi.org/10.1007/s00438-009-0477-z
State	Published - Nov 2009

Bibliographical note

Funding Information:
Acknowledgments This work was supported by grants from the Israel Science Foundation (ISF) and the US–Israel Binational Science Foundation (BSF). M. Xaver was supported by grants F3405 and I031-B of the Austrian science foundation.

Keywords

Chromosome segregation
Homologous recombination
Non-disjunction
Recombination hotspot
YAC DNA
ZIP1

Access to Document

10.1007/s00438-009-0477-z

Cite this

@article{afd7db6f6e7948b187daebdcea01aad4,

title = "Separation of roles of Zip1 in meiosis revealed in heterozygous mutants of Saccharomyces cerevisiae",

abstract = "Synapsis of homologs during meiotic prophase I is associated with a protein complex built along the bivalents-the synaptonemal complex (SC). Mutations in the SC-component gene ZIP1 diminish SC formation, leading to reduced recombination levels and low spore viability. Here we show that in SK1 strains heterozygous for a deletion of ZIP1 in certain regions meiotic interference are impaired with no decrease in recombination levels. The extent of synapsis is over all reduced and NDJ levels of a large endogenous chromosome and of artificial chromosomes (YACs) rise to twice the level of wild type strains. A substantial proportion of mis-segregating YACs had undergone crossing over. This demonstrates that different functions of Zip1 display differential sensitivities to changes in expression levels.",

keywords = "Chromosome segregation, Homologous recombination, Non-disjunction, Recombination hotspot, YAC DNA, ZIP1",

author = "Michael Klutstein and Martin Xaver and Ronen Shemesh and Drora Zenvirth and Franz Klein and Giora Simchen",

note = "Funding Information: Acknowledgments This work was supported by grants from the Israel Science Foundation (ISF) and the US–Israel Binational Science Foundation (BSF). M. Xaver was supported by grants F3405 and I031-B of the Austrian science foundation.",

year = "2009",

month = nov,

doi = "10.1007/s00438-009-0477-z",

language = "אנגלית",

volume = "282",

pages = "453--462",

journal = "Molecular Genetics and Genomics",

issn = "1617-4615",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "5",

}

TY - JOUR

T1 - Separation of roles of Zip1 in meiosis revealed in heterozygous mutants of Saccharomyces cerevisiae

AU - Klutstein, Michael

AU - Xaver, Martin

AU - Shemesh, Ronen

AU - Zenvirth, Drora

AU - Klein, Franz

AU - Simchen, Giora

N1 - Funding Information: Acknowledgments This work was supported by grants from the Israel Science Foundation (ISF) and the US–Israel Binational Science Foundation (BSF). M. Xaver was supported by grants F3405 and I031-B of the Austrian science foundation.

PY - 2009/11

Y1 - 2009/11

N2 - Synapsis of homologs during meiotic prophase I is associated with a protein complex built along the bivalents-the synaptonemal complex (SC). Mutations in the SC-component gene ZIP1 diminish SC formation, leading to reduced recombination levels and low spore viability. Here we show that in SK1 strains heterozygous for a deletion of ZIP1 in certain regions meiotic interference are impaired with no decrease in recombination levels. The extent of synapsis is over all reduced and NDJ levels of a large endogenous chromosome and of artificial chromosomes (YACs) rise to twice the level of wild type strains. A substantial proportion of mis-segregating YACs had undergone crossing over. This demonstrates that different functions of Zip1 display differential sensitivities to changes in expression levels.

AB - Synapsis of homologs during meiotic prophase I is associated with a protein complex built along the bivalents-the synaptonemal complex (SC). Mutations in the SC-component gene ZIP1 diminish SC formation, leading to reduced recombination levels and low spore viability. Here we show that in SK1 strains heterozygous for a deletion of ZIP1 in certain regions meiotic interference are impaired with no decrease in recombination levels. The extent of synapsis is over all reduced and NDJ levels of a large endogenous chromosome and of artificial chromosomes (YACs) rise to twice the level of wild type strains. A substantial proportion of mis-segregating YACs had undergone crossing over. This demonstrates that different functions of Zip1 display differential sensitivities to changes in expression levels.

KW - Chromosome segregation

KW - Homologous recombination

KW - Non-disjunction

KW - Recombination hotspot

KW - YAC DNA

KW - ZIP1

UR - http://www.scopus.com/inward/record.url?scp=72849106318&partnerID=8YFLogxK

U2 - 10.1007/s00438-009-0477-z

DO - 10.1007/s00438-009-0477-z

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 19714362

AN - SCOPUS:72849106318

SN - 1617-4615

VL - 282

SP - 453

EP - 462

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

IS - 5

ER -

Separation of roles of Zip1 in meiosis revealed in heterozygous mutants of Saccharomyces cerevisiae

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this