Separation of roles of Zip1 in meiosis revealed in heterozygous mutants of Saccharomyces cerevisiae

Michael Klutstein*, Martin Xaver, Ronen Shemesh, Drora Zenvirth, Franz Klein, Giora Simchen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Synapsis of homologs during meiotic prophase I is associated with a protein complex built along the bivalents-the synaptonemal complex (SC). Mutations in the SC-component gene ZIP1 diminish SC formation, leading to reduced recombination levels and low spore viability. Here we show that in SK1 strains heterozygous for a deletion of ZIP1 in certain regions meiotic interference are impaired with no decrease in recombination levels. The extent of synapsis is over all reduced and NDJ levels of a large endogenous chromosome and of artificial chromosomes (YACs) rise to twice the level of wild type strains. A substantial proportion of mis-segregating YACs had undergone crossing over. This demonstrates that different functions of Zip1 display differential sensitivities to changes in expression levels.

Original languageAmerican English
Pages (from-to)453-462
Number of pages10
JournalMolecular Genetics and Genomics
Volume282
Issue number5
DOIs
StatePublished - Nov 2009

Bibliographical note

Funding Information:
Acknowledgments This work was supported by grants from the Israel Science Foundation (ISF) and the US–Israel Binational Science Foundation (BSF). M. Xaver was supported by grants F3405 and I031-B of the Austrian science foundation.

Keywords

  • Chromosome segregation
  • Homologous recombination
  • Non-disjunction
  • Recombination hotspot
  • YAC DNA
  • ZIP1

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