Sequence rearrangements may alter the in vivo superhelicity of recombinant plasmids

O. Amster*, A. Zamir

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Electrophoretic resolution of topoisomers was used to compare the in vivo superhelicity of recombinant plasmids containing a fragment of cDNA for an immunoglobulin light chain, cloned in the two possible orientations into the BamHI site of pBR313 or pBR322. Previously, frequent transpositions of IS1 or IS5 were observed into the sequence upstream to the cloned fragment in recombinants in one orientation [(+) plasmids] but not in recombinants in the opposite, (-) orientation [(1982) Nucleic Acids Res. 10, 4525-4542]. The results of the present analyses show that, on average, (-) plasmids are less negatively supercoiled than (+) plasmids, or pBR322. These results suggest that primary sequence rearrangements in plasmids could affect their in vivo topological state, and consequently, perhaps, their effectiveness as recipients of transposable elements.

Original languageAmerican English
Pages (from-to)93-98
Number of pages6
JournalFEBS Letters
Volume197
Issue number1-2
DOIs
StatePublished - 3 Mar 1986

Keywords

  • DNA sequence rearrangement Transposition (E. coli) DNA supercoiling

Fingerprint

Dive into the research topics of 'Sequence rearrangements may alter the in vivo superhelicity of recombinant plasmids'. Together they form a unique fingerprint.

Cite this