Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants

Y. Moskovitz; D. E. Goszczynski; L. Bir; A. Fingstein; H. Czosnek; M. Mawassi

doi:10.1007/s00705-007-1075-1

Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants

Y. Moskovitz, D. E. Goszczynski, L. Bir, A. Fingstein, H. Czosnek, M. Mawassi^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Grapevine virus B (GVB) has been found associated with corky bark-diseased vines. Although the sequence of a 7.6-kb cDNA clone from a GVB isolate from Italy has been described, striking differences in sequences between GVB isolates prompted us to construct an additional full-length GVB clone from the isolate 94/971 and to determine its complete sequence. The cDNA of GVB 94/971 shared a nucleotide sequence identity of only 77% with the GVB isolate from Italy. The cDNA of GVB 94/971 was infectious on Nicotiana plants as demonstrated by symptoms and by means of Northern blot, Western blot and electron microscopic analyses.

Original language	English
Pages (from-to)	323-328
Number of pages	6
Journal	Archives of Virology
Volume	153
Issue number	2
DOIs	https://doi.org/10.1007/s00705-007-1075-1
State	Published - Feb 2008

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abstract = "Grapevine virus B (GVB) has been found associated with corky bark-diseased vines. Although the sequence of a 7.6-kb cDNA clone from a GVB isolate from Italy has been described, striking differences in sequences between GVB isolates prompted us to construct an additional full-length GVB clone from the isolate 94/971 and to determine its complete sequence. The cDNA of GVB 94/971 shared a nucleotide sequence identity of only 77% with the GVB isolate from Italy. The cDNA of GVB 94/971 was infectious on Nicotiana plants as demonstrated by symptoms and by means of Northern blot, Western blot and electron microscopic analyses.",

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AU - Czosnek, H.

AU - Mawassi, M.

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Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants

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