Sequencing and synthesis of pardaxin, a polypeptide from the Red Sea Moses sole with ionophore activity

Yechiel Shai; Jay Fox; Carlo Caratsch; Yu Liang Shih; Charles Edwards; Philip Lazarovici

doi:10.1016/0014-5793(88)81007-X

Sequencing and synthesis of pardaxin, a polypeptide from the Red Sea Moses sole with ionophore activity

Yechiel Shai^*, Jay Fox, Carlo Caratsch, Yu Liang Shih, Charles Edwards, Philip Lazarovici

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

109 Scopus citations

Abstract

Pardaxin, an amphipathic polypeptide secreted by the Red Sea flatfish Pardachirus marmoratus whose sequence is NH₂-G-F-F-A-L-I-P-K-I-I-S-S-P-L-F-K-T-L-L-S-A-V-G-S-A-L-S-S-S-G-G-Q-E, was synthesized by the solid-phase method. The structure was verified by sequencing. The synthetic polypeptide changed the resistance of lipid bilayers by forming pores. At 10^-7-10^-8 M, the synthetic pardaxin increased the frequency of the spontaneous release of quanta of acetylcholine at the neuromuscular junction by up to 100-fold, resembling the native product. Synthetic pardaxin seems to be a suitable tool for investigating the molecular structures underlying channel selectivity.

Original language	English
Pages (from-to)	161-166
Number of pages	6
Journal	FEBS Letters
Volume	242
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(88)81007-X
State	Published - 19 Dec 1988
Externally published	Yes

Keywords

Neuromuscular junction
Pardaxin
Planar lipid bilayer
Pore activity
Presynaptic activity

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10.1016/0014-5793(88)81007-X

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@article{a8898d40c097449096d430ac03450191,

title = "Sequencing and synthesis of pardaxin, a polypeptide from the Red Sea Moses sole with ionophore activity",

abstract = "Pardaxin, an amphipathic polypeptide secreted by the Red Sea flatfish Pardachirus marmoratus whose sequence is NH2-G-F-F-A-L-I-P-K-I-I-S-S-P-L-F-K-T-L-L-S-A-V-G-S-A-L-S-S-S-G-G-Q-E, was synthesized by the solid-phase method. The structure was verified by sequencing. The synthetic polypeptide changed the resistance of lipid bilayers by forming pores. At 10-7-10-8 M, the synthetic pardaxin increased the frequency of the spontaneous release of quanta of acetylcholine at the neuromuscular junction by up to 100-fold, resembling the native product. Synthetic pardaxin seems to be a suitable tool for investigating the molecular structures underlying channel selectivity.",

keywords = "Neuromuscular junction, Pardaxin, Planar lipid bilayer, Pore activity, Presynaptic activity",

author = "Yechiel Shai and Jay Fox and Carlo Caratsch and Shih, {Yu Liang} and Charles Edwards and Philip Lazarovici",

year = "1988",

month = dec,

day = "19",

doi = "10.1016/0014-5793(88)81007-X",

language = "אנגלית",

volume = "242",

pages = "161--166",

journal = "FEBS Letters",

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publisher = "John Wiley and Sons Inc.",

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AU - Shai, Yechiel

AU - Fox, Jay

AU - Caratsch, Carlo

AU - Shih, Yu Liang

AU - Edwards, Charles

AU - Lazarovici, Philip

PY - 1988/12/19

Y1 - 1988/12/19

N2 - Pardaxin, an amphipathic polypeptide secreted by the Red Sea flatfish Pardachirus marmoratus whose sequence is NH2-G-F-F-A-L-I-P-K-I-I-S-S-P-L-F-K-T-L-L-S-A-V-G-S-A-L-S-S-S-G-G-Q-E, was synthesized by the solid-phase method. The structure was verified by sequencing. The synthetic polypeptide changed the resistance of lipid bilayers by forming pores. At 10-7-10-8 M, the synthetic pardaxin increased the frequency of the spontaneous release of quanta of acetylcholine at the neuromuscular junction by up to 100-fold, resembling the native product. Synthetic pardaxin seems to be a suitable tool for investigating the molecular structures underlying channel selectivity.

AB - Pardaxin, an amphipathic polypeptide secreted by the Red Sea flatfish Pardachirus marmoratus whose sequence is NH2-G-F-F-A-L-I-P-K-I-I-S-S-P-L-F-K-T-L-L-S-A-V-G-S-A-L-S-S-S-G-G-Q-E, was synthesized by the solid-phase method. The structure was verified by sequencing. The synthetic polypeptide changed the resistance of lipid bilayers by forming pores. At 10-7-10-8 M, the synthetic pardaxin increased the frequency of the spontaneous release of quanta of acetylcholine at the neuromuscular junction by up to 100-fold, resembling the native product. Synthetic pardaxin seems to be a suitable tool for investigating the molecular structures underlying channel selectivity.

KW - Neuromuscular junction

KW - Pardaxin

KW - Planar lipid bilayer

KW - Pore activity

KW - Presynaptic activity

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JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Sequencing and synthesis of pardaxin, a polypeptide from the Red Sea Moses sole with ionophore activity

Abstract

Keywords

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