Serological and molecular survey of Leishmania infection in dogs from Luanda, Angola

Hugo Vilhena, Sara Granada, Ana Cristina Oliveira, Henk Dfh Schallig, Yaarit Nachum-Biala, Luís Cardoso*, Gad Baneth

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Background: Canine leishmaniosis (CanL) due to Leishmania infantum is a global zoonosis endemic in more than 70 countries in Europe, North Africa, Asia and America; however, data on this infection is scarce from southern Africa. The aim of this study was to survey dogs in Luanda, Angola, for Leishmania infection. Findings. One hundred-and-three dogs presented to a veterinary medical centre in Luanda were serologically and molecularly assessed for Leishmania with the direct agglutination test (DAT) and polymerase chain reaction (PCR). Two dogs were seropositive, with DAT titres of 800 and ≥6400; the latter was also found to be PCR-positive and confirmed to be infected with L. infantum by DNA sequence analysis. No other dog was found to be PCR-positive. The first dog had been imported from Portugal, but the latter had never left Angola (neither had its parents), strongly suggesting an autochthonous infection. Conclusions: Although other cases of CanL have previously been described in the country, this is the first reported study of canine Leishmania infection at the population level, as well as the first report on the molecular characterization of L. infantum in dogs from Angola.

Original languageAmerican English
Article number114
JournalParasites and Vectors
Issue number1
StatePublished - 24 Mar 2014


  • Angola
  • Canine leishmaniosis
  • Direct agglutination test
  • Dogs
  • Leishmania infantum
  • Luanda
  • Polymerase chain reaction


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