Serosal mast cells maintain their viability and promote the metabolism of cartilage proteoglycans when cocultured with chondrocytes

Richard L. Stevens*, Laura L. Somerville, Duane Sewell, James R. Swafford, John P. Caulfield, Francesca Levi‐Schaffer, John R. Hubbard, Elahe T. Dayton

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Objective. To determine the consequences of mast cell (MC)–chondrocyte interactions. Methods. Cocultured cells were analyzed histochemically, morphologically, biochemically, and functionally. Results. Cocultured MC adhered to the chondrocytes and remained viable. Chondrocytes cocultured with nonactivated MC produced more proteoglycans than did chondrocytes cultured alone, and these proteoglycans possessed an intact hyaluronic acid—binding region. In contrast, most of the proteoglycans produced by chondrocytes cocultured with activated MC were degraded. Conclusion. These studies indicate that a complex interaction occurs in which the nonactivated MC stimulates biosynthesis and the activated MC degrades cartilage proteoglycans.

Original languageAmerican English
Pages (from-to)325-335
Number of pages11
JournalArthritis and Rheumatology
Volume35
Issue number3
DOIs
StatePublished - Mar 1992

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