Sfp1 is a stress- and nutrient-sensitive regulator of ribosomal protein gene expression

Rosa M. Marion, Aviv Regev, Eran Segal, Yoseph Barash, Daphne Koller, Nir Friedman, Erin K. O'Shea*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

283 Scopus citations

Abstract

Yeast cells modulate their protein synthesis capacity in response to physiological needs through the transcriptional control of ribosomal protein (RP) genes. Here we demonstrate that the transcription factor Sfp1, previously shown to play a role in the control of cell size, regulates RP gene expression in response to nutrients and stress. Under optimal growth conditions, Sfp1 is localized to the nucleus, bound to the promoters of RP genes, and helps promote RP gene expression. In response to inhibition of target of rapamycin (TOR) signaling, stress, or changes in nutrient availability, Sfp1 is released from RP gene promoters and leaves the nucleus, and RP gene transcription is down-regulated. Additionally, cells lacking Sfp1 fail to appropriately modulate RP gene expression in response to environmental cues. We conclude that Sfp1 integrates information from nutrient- and stress-responsive signaling pathways to help control RP gene expression.

Original languageAmerican English
Pages (from-to)14315-14322
Number of pages8
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number40
DOIs
StatePublished - 5 Oct 2004

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