Simultaneous Mapping of Enhancers and Enhancer Rearrangements with Paired-End H3K27ac ChIP-seq

Israel Antman, Ester Davis, Shahd Abu-Kamel, Merav Hecht, Yotam Drier*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations


We describe a protocol for H3K27ac ChIP paired-end sequencing and computational analysis of rearrangements. Our approach can be used to simultaneously map enhancers and their activity and to identify structural variations at enhancers. Since changes in enhancer activity and new enhancer translocations both play a major role in tumor initiation, progression, and response to therapy, this approach holds promise to uncover some of the mechanisms behind these processes.

Original languageAmerican English
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


  • ChIP-seq
  • Enhancer rearrangements
  • Histone modifications
  • Structural variation


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