Simultaneous Mapping of Enhancers and Enhancer Rearrangements with Paired-End H3K27ac ChIP-seq

Israel Antman; Ester Davis; Shahd Abu-Kamel; Merav Hecht; Yotam Drier

doi:10.1007/978-1-0716-2513-2_11

Simultaneous Mapping of Enhancers and Enhancer Rearrangements with Paired-End H3K27ac ChIP-seq

Israel Antman, Ester Davis, Shahd Abu-Kamel, Merav Hecht, Yotam Drier^*

^*Corresponding author for this work

Department of Immunology and Cancer Research

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

We describe a protocol for H3K27ac ChIP paired-end sequencing and computational analysis of rearrangements. Our approach can be used to simultaneously map enhancers and their activity and to identify structural variations at enhancers. Since changes in enhancer activity and new enhancer translocations both play a major role in tumor initiation, progression, and response to therapy, this approach holds promise to uncover some of the mechanisms behind these processes.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	131-140
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-0716-2513-2_11
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2535
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

ChIP-seq
Enhancer rearrangements
Histone modifications
Structural variation

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10.1007/978-1-0716-2513-2_11

Cite this

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AU - Hecht, Merav

AU - Drier, Yotam

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Simultaneous Mapping of Enhancers and Enhancer Rearrangements with Paired-End H3K27ac ChIP-seq

Abstract

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Bibliographical note

Keywords

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