Simultaneous measurement of oxygen, carbon dioxide, and water vapour exchange in intact plants

A. Kaplan*, J. Gale, Alexandra Poljakoff-mayber

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A low flow of air is passed through a temperature-controlled plant chamber in order to obtain relatively large (∼300-500 μl-1) differences in [O2] between influx and efflux streams. These differences are measured with a stabilized O2 electrode system incorporating elements of gas conditioning electronic zero suppression and signal amplification. Changes in [O2] of 400 μl l-1 can be detected at full scale recorder deflection against a background concentration of 21% O2. The concentrations of CO2 and H2O within the chamber are held constant by con-trolled-flow CO2-scrubbing and dehumidifying loops. Carbon dioxide, H2O, and O2 fluxes are measured and leaf diffusion resistance and internal [CO2] are calculated in essentially 'real time'.

Original languageEnglish
Pages (from-to)214-219
Number of pages6
JournalJournal of Experimental Botany
Volume27
Issue number2
DOIs
StatePublished - Apr 1976

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