Simultaneous measurement of oxygen, carbon dioxide, and water vapour exchange in intact plants

A. Kaplan; J. Gale; Alexandra Poljakoff-mayber

doi:10.1093/jxb/27.2.214

Simultaneous measurement of oxygen, carbon dioxide, and water vapour exchange in intact plants

A. Kaplan^*
, J. Gale
, Alexandra Poljakoff-mayber

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A low flow of air is passed through a temperature-controlled plant chamber in order to obtain relatively large (∼300-500 μl-1) differences in [O₂] between influx and efflux streams. These differences are measured with a stabilized O₂ electrode system incorporating elements of gas conditioning electronic zero suppression and signal amplification. Changes in [O₂] of 400 μl l-1 can be detected at full scale recorder deflection against a background concentration of 21% O₂. The concentrations of CO₂ and H₂O within the chamber are held constant by con-trolled-flow CO₂-scrubbing and dehumidifying loops. Carbon dioxide, H₂O, and O₂ fluxes are measured and leaf diffusion resistance and internal [CO₂] are calculated in essentially 'real time'.

Original language	English
Pages (from-to)	214-219
Number of pages	6
Journal	Journal of Experimental Botany
Volume	27
Issue number	2
DOIs	https://doi.org/10.1093/jxb/27.2.214
State	Published - Apr 1976

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10.1093/jxb/27.2.214

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title = "Simultaneous measurement of oxygen, carbon dioxide, and water vapour exchange in intact plants",

abstract = "A low flow of air is passed through a temperature-controlled plant chamber in order to obtain relatively large (∼300-500 μl-1) differences in [O2] between influx and efflux streams. These differences are measured with a stabilized O2 electrode system incorporating elements of gas conditioning electronic zero suppression and signal amplification. Changes in [O2] of 400 μl l-1 can be detected at full scale recorder deflection against a background concentration of 21\% O2. The concentrations of CO2 and H2O within the chamber are held constant by con-trolled-flow CO2-scrubbing and dehumidifying loops. Carbon dioxide, H2O, and O2 fluxes are measured and leaf diffusion resistance and internal [CO2] are calculated in essentially 'real time'.",

author = "A. Kaplan and J. Gale and Alexandra Poljakoff-mayber",

year = "1976",

month = apr,

doi = "10.1093/jxb/27.2.214",

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journal = "Journal of Experimental Botany",

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T1 - Simultaneous measurement of oxygen, carbon dioxide, and water vapour exchange in intact plants

AU - Kaplan, A.

AU - Gale, J.

AU - Poljakoff-mayber, Alexandra

PY - 1976/4

Y1 - 1976/4

N2 - A low flow of air is passed through a temperature-controlled plant chamber in order to obtain relatively large (∼300-500 μl-1) differences in [O2] between influx and efflux streams. These differences are measured with a stabilized O2 electrode system incorporating elements of gas conditioning electronic zero suppression and signal amplification. Changes in [O2] of 400 μl l-1 can be detected at full scale recorder deflection against a background concentration of 21% O2. The concentrations of CO2 and H2O within the chamber are held constant by con-trolled-flow CO2-scrubbing and dehumidifying loops. Carbon dioxide, H2O, and O2 fluxes are measured and leaf diffusion resistance and internal [CO2] are calculated in essentially 'real time'.

AB - A low flow of air is passed through a temperature-controlled plant chamber in order to obtain relatively large (∼300-500 μl-1) differences in [O2] between influx and efflux streams. These differences are measured with a stabilized O2 electrode system incorporating elements of gas conditioning electronic zero suppression and signal amplification. Changes in [O2] of 400 μl l-1 can be detected at full scale recorder deflection against a background concentration of 21% O2. The concentrations of CO2 and H2O within the chamber are held constant by con-trolled-flow CO2-scrubbing and dehumidifying loops. Carbon dioxide, H2O, and O2 fluxes are measured and leaf diffusion resistance and internal [CO2] are calculated in essentially 'real time'.

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JO - Journal of Experimental Botany

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IS - 2

ER -

Simultaneous measurement of oxygen, carbon dioxide, and water vapour exchange in intact plants

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