Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome

Antony B. Holmes; Clarissa Corinaldesi; Qiong Shen; Rahul Kumar; Nicolo Compagno; Zhong Wang; Mor Nitzan; Eli Grunstein; Laura Pasqualucci; Riccardo Dalla-Favera; Katia Basso

doi:10.1084/JEM.20200483

Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome

Antony B. Holmes, Clarissa Corinaldesi, Qiong Shen, Rahul Kumar, Nicolo Compagno, Zhong Wang, Mor Nitzan, Eli Grunstein, Laura Pasqualucci, Riccardo Dalla-Favera^*, Katia Basso^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

123 Scopus citations

Abstract

In response to T cell-dependent antigens, mature B cells are stimulated to form germinal centers (GCs), the sites of B cell affinity maturation and the cell of origin (COO) of most B cell lymphomas. To explore the dynamics of GC B cell development beyond the known dark zone and light zone compartments, we performed single-cell (sc) transcriptomic analysis on human GC B cells and identified multiple functionally linked subpopulations, including the distinct precursors of memory B cells and plasma cells. The gene expression signatures associated with these GC subpopulations were effective in providing a sc-COO for ∼80% of diffuse large B cell lymphomas (DLBCLs) and identified novel prognostic subgroups of DLBCL.

Original language	English
Article number	200483
Journal	Journal of Experimental Medicine
Volume	217
Issue number	10
DOIs	https://doi.org/10.1084/JEM.20200483
State	Published - 2020
Externally published	Yes

Bibliographical note

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© 2020 Rockefeller University Press. All rights reserved.

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abstract = "In response to T cell-dependent antigens, mature B cells are stimulated to form germinal centers (GCs), the sites of B cell affinity maturation and the cell of origin (COO) of most B cell lymphomas. To explore the dynamics of GC B cell development beyond the known dark zone and light zone compartments, we performed single-cell (sc) transcriptomic analysis on human GC B cells and identified multiple functionally linked subpopulations, including the distinct precursors of memory B cells and plasma cells. The gene expression signatures associated with these GC subpopulations were effective in providing a sc-COO for ∼80% of diffuse large B cell lymphomas (DLBCLs) and identified novel prognostic subgroups of DLBCL.",

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AU - Holmes, Antony B.

AU - Corinaldesi, Clarissa

AU - Shen, Qiong

AU - Kumar, Rahul

AU - Compagno, Nicolo

AU - Wang, Zhong

AU - Nitzan, Mor

AU - Grunstein, Eli

AU - Pasqualucci, Laura

AU - Dalla-Favera, Riccardo

AU - Basso, Katia

PY - 2020

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AB - In response to T cell-dependent antigens, mature B cells are stimulated to form germinal centers (GCs), the sites of B cell affinity maturation and the cell of origin (COO) of most B cell lymphomas. To explore the dynamics of GC B cell development beyond the known dark zone and light zone compartments, we performed single-cell (sc) transcriptomic analysis on human GC B cells and identified multiple functionally linked subpopulations, including the distinct precursors of memory B cells and plasma cells. The gene expression signatures associated with these GC subpopulations were effective in providing a sc-COO for ∼80% of diffuse large B cell lymphomas (DLBCLs) and identified novel prognostic subgroups of DLBCL.

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Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome

Abstract

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