Single-Cell Resolution Optogenetics Via Expression of Soma-Targeted Rhodopsins

Changyang Linghu; I. Wen Chen; Dimitrii Tanese; Valeria Zampini; Or A. Shemesh

doi:10.1007/978-1-0716-2329-9_11

Single-Cell Resolution Optogenetics Via Expression of Soma-Targeted Rhodopsins

Changyang Linghu, I. Wen Chen, Dimitrii Tanese, Valeria Zampini, Or A. Shemesh^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Optogenetics allows control of neural activity in genetically targeted neuron populations by light. Optogenetic control of individual neurons in neural circuits would enable powerful, causal investigations of neural connectivity and function at single-cell level and provide insights into how neural circuits operate. Such single-cell resolution optogenetics in neuron populations requires precise sculpting of light and subcellular targeting of optogenetic molecules. Here we describe a group of methods for single-cell resolution optogenetics in neuron cultures, in mouse brain slices, and in mouse cortex in-vivo, via patterned light and soma-targeted optogenetic molecules.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	229-257
Number of pages	29
DOIs	https://doi.org/10.1007/978-1-0716-2329-9_11
State	Published - 2022
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2501
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Cell-body-targeted opsins
Computer-generated holography
Functional connectivity
Single-cell control
Single-cell optogenetics
Somatic optogenetics
Synaptic transmission
Two-photon stimulation

Access to Document

10.1007/978-1-0716-2329-9_11

Cite this

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Single-Cell Resolution Optogenetics Via Expression of Soma-Targeted Rhodopsins

Abstract

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Bibliographical note

Keywords

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