Single-Cell Transcriptomics Identifies the Adaptation of Scart1+ Vγ6+ T Cells to Skin Residency as Activated Effector Cells

Likai Tan, Inga Sandrock, Ivan Odak, Yuval Aizenbud, Anneke Wilharm, Joana Barros-Martins, Yaara Tabib, Alina Borchers, Tiago Amado, Lahiru Gangoda, Marco J. Herold, Marc Schmidt-Supprian, Jan Kisielow, Bruno Silva-Santos, Christian Koenecke, Avi Hai Hovav, Christian Krebs, Immo Prinz*, Sarina Ravens

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


IL-17-producing γδ T cells express oligoclonal Vγ4+ and Vγ6+ TCRs, mainly develop in the prenatal thymus, and later persist as long-lived self-renewing cells in all kinds of tissues. However, their exchange between tissues and the mechanisms of their tissue-specific adaptation remain poorly understood. Here, single-cell RNA-seq profiling identifies IL-17-producing Vγ6+ T cells as a highly homogeneous Scart1+ population in contrast to their Scart2+ IL-17-producing Vγ4+ T cell counterparts. Parabiosis demonstrates that Vγ6+ T cells are fairly tissue resident in the thymus, peripheral lymph nodes, and skin. There, Scart1+ Vγ6+ T cells display tissue-specific gene expression signatures in the skin, characterized by steady-state production of the cytokines IL-17A and amphiregulin as well as by high expression of the anti-apoptotic Bcl2a1 protein family. Together, this study demonstrates how Scart1+ Vγ6+ T cells undergo tissue-specific functional adaptation to persist as effector cells in their skin habitat. Tan et al. apply single-cell transcriptome analysis of tissue-resident Vγ6+ T cells and identify molecular determinants that mediate the functional adaptation and longevity of Scart1+ Vγ6+ T cells within tissues.

Original languageAmerican English
Pages (from-to)3657-3671.e4
JournalCell Reports
Issue number12
StatePublished - 18 Jun 2019

Bibliographical note

Publisher Copyright:
© 2019 The Author(s)


  • IL-17-producing γδ T cells
  • Scart1
  • Scart2
  • Vγ6 T cells
  • single-cell transcriptomics
  • tissue-resident γδ T cells


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