Single-pulse CARS based multimodal nonlinear optical microscope for bioimaging

Sunil Kumar, Tschackad Kamali, Jonathan M. Levitte, Ori Katz, Boris Hermann, Rene Werkmeister, Boris Považay, Wolfgang Drexler, Angelika Unterhuber, Yaron Silberberg

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Noninvasive label-free imaging of biological systems raises demand not only for high-speed three-dimensional prescreening of morphology over a wide-field of view but also it seeks to extract the microscopic functional and molecular details within. Capitalizing on the unique advantages brought out by different nonlinear optical effects, a multimodal nonlinear optical microscope can be a powerful tool for bioimaging. Bringing together the intensity-dependent contrast mechanisms via second harmonic generation, third harmonic generation and four-wave mixing for structural-sensitive imaging, and single-beam/single-pulse coherent anti-Stokes Raman scattering technique for chemical sensitive imaging in the finger-print region, we have developed a simple and nearly alignment-free multimodal nonlinear optical microscope that is based on a single wide-band Ti:Sapphire femtosecond pulse laser source. Successful imaging tests have been realized on two exemplary biological samples, a canine femur bone and collagen fibrils harvested from a rat tail. Since the ultra-broad band-width femtosecond laser is a suitable source for performing high-resolution optical coherence tomography, a wide-field optical coherence tomography arm can be easily incorporated into the presented multimodal microscope making it a versatile optical imaging tool for noninvasive label-free bioimaging.

Original languageAmerican English
Pages (from-to)13082-13098
Number of pages17
JournalOptics Express
Issue number10
StatePublished - 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
©2015 Optical Society of America.


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