Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022

Pnina Gottfried, Ofra Lotan, Mikhail Kolot, Ludmila Maslenin, Rosa Bendov, Rena Gorovits, Vered Yesodi, Ezra Yagil*, Arie Rosner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP × attB) as well as a site-specific excision reaction (attL × attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization.

Original languageAmerican English
Pages (from-to)435-444
Number of pages10
JournalPlant Molecular Biology
Volume57
Issue number3
DOIs
StatePublished - Feb 2005

Keywords

  • Arabidopsis
  • Integrase
  • Nuclear localization
  • Phage HK022
  • Site-specific recombination

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