Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022

Pnina Gottfried; Ofra Lotan; Mikhail Kolot; Ludmila Maslenin; Rosa Bendov; Rena Gorovits; Vered Yesodi; Ezra Yagil; Arie Rosner

doi:10.1007/s11103-004-0076-7

Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022

Pnina Gottfried, Ofra Lotan, Mikhail Kolot, Ludmila Maslenin, Rosa Bendov, Rena Gorovits, Vered Yesodi, Ezra Yagil^*, Arie Rosner

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP × attB) as well as a site-specific excision reaction (attL × attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization.

Original language	English
Pages (from-to)	435-444
Number of pages	10
Journal	Plant Molecular Biology
Volume	57
Issue number	3
DOIs	https://doi.org/10.1007/s11103-004-0076-7
State	Published - Feb 2005

Keywords

Arabidopsis
Integrase
Nuclear localization
Phage HK022
Site-specific recombination

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10.1007/s11103-004-0076-7

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abstract = "The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP × attB) as well as a site-specific excision reaction (attL × attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization.",

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AU - Gottfried, Pnina

AU - Lotan, Ofra

AU - Kolot, Mikhail

AU - Maslenin, Ludmila

AU - Bendov, Rosa

AU - Gorovits, Rena

AU - Yesodi, Vered

AU - Yagil, Ezra

AU - Rosner, Arie

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KW - Arabidopsis

KW - Integrase

KW - Nuclear localization

KW - Phage HK022

KW - Site-specific recombination

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Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022

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