Sol-gel derived, ferrocenyl-modified silicate-graphite composite electrode: Wiring of glucose oxidase

J. Gun, O. Lev*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

88 Scopus citations


A new type of porous, hybrid organic-inorganic material is synthesized and used for direct wiring of active enzymes. The material witnesses a dispersion of graphite powder and redox enzyme incorporated in a multi-functional, ferrocene-, amine- and methyl-modified silicate backbone. Each species in this integrated construction accomplishes a specialized task: (a) the graphite provides conductivity by percolation; (b) the silicate provides the highly crosslinked, rigid backbone which is used to cage the redox enzyme; (c) ferrocene functional groups are responsible for the signal transduction from the active center of the enzyme to the electron conductive surface; (d) amine groups are incorporated for their high affinity for excess negative charges on the surface of glucose oxidase. Also, the combination of methyl and amine groups is advantageous to maintain control over the wetted electroactive section of the electrode. Amperometric sensing of glucose demonstrates the application of this new material.

Original languageAmerican English
Pages (from-to)95-106
Number of pages12
JournalAnalytica Chimica Acta
Issue number1-3
StatePublished - 30 Dec 1996

Bibliographical note

Funding Information:
This research was supportedb y a grant from the Ministry of Science and the Arts of Israel and by the GBF-Gesellschaft Fuer Biotechnologische For-schung, MBH, Braunschweig, Germany. We gratefully acknowledge useful discussions with M. Dvorchak, I. Kuselman, B. Anisimov, B. Ginzburg, S. Sampatha nd A. Turniansky.


  • Composite ceramic-carbon electrodes
  • Enzymatic methods
  • Ferrocenyl modified silicate


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