Sol-gel entrapment of monoclonal anti-atrazine antibodies

A. Turniansky*, D. Avnir, A. Bronshtein, N. Aharonson, M. Altstein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

We report the successful doping of a sol-gel matrix with an antibody, retaining its ability to bind free antigen from an aqueous solution. The particular system described is monoclonal anti-atrazine mouse antibody which was entrapped in SiO2 sol-gel matrices, prepared from tetramethoxysilane by several methods. Atrazine was selected as a model compound for this study, within the framework of the development of immunochemical-based methods for monitoring pesticide residues and other organo-synthetic environmental contaminants. Nanogram quantities of atrazine were applied on SiO2 sol-gel columns doped with this antibody, and the amount of eluted antigen was determined by Enzyme Linked ImmunoSorbent Assay (ELISA). Under appropriate sol-gel-forming conditions, high amounts of atrazine were bound to the sol-gels, ranging between 60% and 91% of the amount applied to the column. The combination of the properties of the sol-gel matrix (e.g., stability, inertness, high porosity, high surface area and optical clarity), together with the selectivity and sensitivity of the antibodies, enable extension of this feasibility study to development of a novel group of immunosensors which could be used for purification, concentration and monitoring of a variety of residues from different sources.

Original languageEnglish
Pages (from-to)135-143
Number of pages9
JournalJournal of Sol-Gel Science and Technology
Volume7
Issue number1-2
DOIs
StatePublished - 1996

Keywords

  • Antibody
  • Atrazine
  • Chromatography
  • ELISA
  • Entrapment
  • Immunoassay
  • Pesticide residue
  • Sol-gel

Fingerprint

Dive into the research topics of 'Sol-gel entrapment of monoclonal anti-atrazine antibodies'. Together they form a unique fingerprint.

Cite this