Sol-gel luminescence biosensors: Encapsulation of recombinant E. coli reporters in thick silicate films

J. Rajan Premkumar; Rachel Rosen; Shimshon Belkin; Ovadia Lev

doi:10.1016/S0003-2670(02)00301-X

Sol-gel luminescence biosensors: Encapsulation of recombinant E. coli reporters in thick silicate films

J. Rajan Premkumar, Rachel Rosen, Shimshon Belkin, Ovadia Lev^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

105 Scopus citations

Abstract

A class of sensing elements based on the encapsulation of genetically engineered bioluminescent Escherichia coli (E. coli) reporter strains in sol-gel derived silicates is described. We demonstrate the concept by the immobilization of these bacterial cells in thick silicate films. Heat shock, oxidative stress, fatty acids, peroxides, and genotoxicity reporting bacteria were incorporated in the sol-gel silicates and their luminescence response was compared to that of the non-immobilized culture. All the immobilized bacteria maintained viability and luminescence activity for several months. The bacteria-silicate hybrids can be used either as disposable sensors or in multiple use sensing test-kits, and they can be also integrated in early warning devices operated in continuous flow conditions.

Original language	English
Pages (from-to)	11-23
Number of pages	13
Journal	Analytica Chimica Acta
Volume	462
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2670(02)00301-X
State	Published - 26 Jun 2002

Bibliographical note

Funding Information:
This research was supported by the Infrastructure Program of the Ministry of Science, Culture, and Sport, Israel, and by a Franco-Israel research grant; Instrumentation was partly funded by the Israel Science Foundation, founded by the Israel Academy of Sciences. We gratefully acknowledge strains provided by T.K. Van Dyk and R.A. LaRossa, the DuPont Company, USA.

Keywords

Bioluminescence
Biosensors
E. coli
Genotoxicity testing
Sol-gel
Toxicity testing

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10.1016/S0003-2670(02)00301-X

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title = "Sol-gel luminescence biosensors: Encapsulation of recombinant E. coli reporters in thick silicate films",

abstract = "A class of sensing elements based on the encapsulation of genetically engineered bioluminescent Escherichia coli (E. coli) reporter strains in sol-gel derived silicates is described. We demonstrate the concept by the immobilization of these bacterial cells in thick silicate films. Heat shock, oxidative stress, fatty acids, peroxides, and genotoxicity reporting bacteria were incorporated in the sol-gel silicates and their luminescence response was compared to that of the non-immobilized culture. All the immobilized bacteria maintained viability and luminescence activity for several months. The bacteria-silicate hybrids can be used either as disposable sensors or in multiple use sensing test-kits, and they can be also integrated in early warning devices operated in continuous flow conditions.",

keywords = "Bioluminescence, Biosensors, E. coli, Genotoxicity testing, Sol-gel, Toxicity testing",

author = "Premkumar, {J. Rajan} and Rachel Rosen and Shimshon Belkin and Ovadia Lev",

note = "Funding Information: This research was supported by the Infrastructure Program of the Ministry of Science, Culture, and Sport, Israel, and by a Franco-Israel research grant; Instrumentation was partly funded by the Israel Science Foundation, founded by the Israel Academy of Sciences. We gratefully acknowledge strains provided by T.K. Van Dyk and R.A. LaRossa, the DuPont Company, USA.",

year = "2002",

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doi = "10.1016/S0003-2670(02)00301-X",

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T2 - Encapsulation of recombinant E. coli reporters in thick silicate films

AU - Premkumar, J. Rajan

AU - Rosen, Rachel

AU - Belkin, Shimshon

AU - Lev, Ovadia

N1 - Funding Information: This research was supported by the Infrastructure Program of the Ministry of Science, Culture, and Sport, Israel, and by a Franco-Israel research grant; Instrumentation was partly funded by the Israel Science Foundation, founded by the Israel Academy of Sciences. We gratefully acknowledge strains provided by T.K. Van Dyk and R.A. LaRossa, the DuPont Company, USA.

PY - 2002/6/26

Y1 - 2002/6/26

N2 - A class of sensing elements based on the encapsulation of genetically engineered bioluminescent Escherichia coli (E. coli) reporter strains in sol-gel derived silicates is described. We demonstrate the concept by the immobilization of these bacterial cells in thick silicate films. Heat shock, oxidative stress, fatty acids, peroxides, and genotoxicity reporting bacteria were incorporated in the sol-gel silicates and their luminescence response was compared to that of the non-immobilized culture. All the immobilized bacteria maintained viability and luminescence activity for several months. The bacteria-silicate hybrids can be used either as disposable sensors or in multiple use sensing test-kits, and they can be also integrated in early warning devices operated in continuous flow conditions.

AB - A class of sensing elements based on the encapsulation of genetically engineered bioluminescent Escherichia coli (E. coli) reporter strains in sol-gel derived silicates is described. We demonstrate the concept by the immobilization of these bacterial cells in thick silicate films. Heat shock, oxidative stress, fatty acids, peroxides, and genotoxicity reporting bacteria were incorporated in the sol-gel silicates and their luminescence response was compared to that of the non-immobilized culture. All the immobilized bacteria maintained viability and luminescence activity for several months. The bacteria-silicate hybrids can be used either as disposable sensors or in multiple use sensing test-kits, and they can be also integrated in early warning devices operated in continuous flow conditions.

KW - Bioluminescence

KW - Biosensors

KW - E. coli

KW - Genotoxicity testing

KW - Sol-gel

KW - Toxicity testing

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JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

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Sol-gel luminescence biosensors: Encapsulation of recombinant E. coli reporters in thick silicate films

Abstract

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Keywords

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