TY - JOUR
T1 - Solubilization of sphingomyelin by triton X-100 effect of the method of mixing and the concentrations of detergent and lipid
AU - Yedgar, Shaul
AU - Hertz, Rachel
AU - Gatt, Shimon
PY - 1974/12
Y1 - 1974/12
N2 - Mixed dispersions of sphingomyelin and Triton X-100 were prepared by two procedures. In method A, aqueous dispersions of sphingomyelin were mixed with aqueous solutions of Triton X-100. In method B, solutions of sphingomyelin and Triton X-100 in organic solvent were mixed, the solvent was evaporated and the dry residue was dispersed in buffer. Measurement of turbidities, electron microscopy and sedimentation of the mixed dispersions suggested the following: Below the critical micellar concentration of Triton X-100, the sphingomyelin is present as liposomes which sediment in the ultracentrifuge. Above the CMC, mixed micelles of sphingomyelin and Triton form. Method B resulted in aggregates of sphingomyelin which contain Triton X-100 even below its critical micellar concentration and which are smaller than those obtained by method A.
AB - Mixed dispersions of sphingomyelin and Triton X-100 were prepared by two procedures. In method A, aqueous dispersions of sphingomyelin were mixed with aqueous solutions of Triton X-100. In method B, solutions of sphingomyelin and Triton X-100 in organic solvent were mixed, the solvent was evaporated and the dry residue was dispersed in buffer. Measurement of turbidities, electron microscopy and sedimentation of the mixed dispersions suggested the following: Below the critical micellar concentration of Triton X-100, the sphingomyelin is present as liposomes which sediment in the ultracentrifuge. Above the CMC, mixed micelles of sphingomyelin and Triton form. Method B resulted in aggregates of sphingomyelin which contain Triton X-100 even below its critical micellar concentration and which are smaller than those obtained by method A.
UR - http://www.scopus.com/inward/record.url?scp=0016220781&partnerID=8YFLogxK
U2 - 10.1016/0009-3084(74)90013-9
DO - 10.1016/0009-3084(74)90013-9
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C2 - 4452219
AN - SCOPUS:0016220781
SN - 0009-3084
VL - 13
SP - 404
EP - 414
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
IS - 4
ER -