Solubilization of sphingomyelin by triton X-100 effect of the method of mixing and the concentrations of detergent and lipid

Shaul Yedgar*, Rachel Hertz, Shimon Gatt

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Mixed dispersions of sphingomyelin and Triton X-100 were prepared by two procedures. In method A, aqueous dispersions of sphingomyelin were mixed with aqueous solutions of Triton X-100. In method B, solutions of sphingomyelin and Triton X-100 in organic solvent were mixed, the solvent was evaporated and the dry residue was dispersed in buffer. Measurement of turbidities, electron microscopy and sedimentation of the mixed dispersions suggested the following: Below the critical micellar concentration of Triton X-100, the sphingomyelin is present as liposomes which sediment in the ultracentrifuge. Above the CMC, mixed micelles of sphingomyelin and Triton form. Method B resulted in aggregates of sphingomyelin which contain Triton X-100 even below its critical micellar concentration and which are smaller than those obtained by method A.

Original languageEnglish
Pages (from-to)404-414
Number of pages11
JournalChemistry and Physics of Lipids
Volume13
Issue number4
DOIs
StatePublished - Dec 1974

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