TY - JOUR
T1 - Soluble FcγRIIa inhibits rheumatoid factor binding to immune complexes
AU - Wines, Bruce D.
AU - Gavin, Amanda
AU - Powell, Maree S.
AU - Steinitz, Michael
AU - Buchanan, Russell R.C.
AU - Hogarth, P. Mark
PY - 2003/6/1
Y1 - 2003/6/1
N2 - Soluble low-affinity receptors for IgG are known to inhibit immune complex (IC)-mediated inflammation, and expression by leukocytes is elevated in several inflammatory diseases. Immunoglobulin M (IgM) rheumatoid factors (RF), anti-Fc autoantibodies, are found in autoimmune diseases, such as rheumatoid arthritis (RA), as well as in normal immune responses. This study demonstrated that soluble FcγRIIa inhibits the interaction of rheumatoid factors with ICs. The recombinant soluble low-affinity FcγR, rsFcγRIIa, partially inhibited (30-70%) the rate of precipitation of soluble ICs by RF-positive RA sera. This required the normal interaction of FcγRIIa with Fc as the effect could be abrogated with the Fab fragment of the blocking mAb IV-3. Furthermore, rsFcγRIIa partially inhibited (40%) the binding of a monoclonal IgM RF (RF-AN) to an IC formed by IgG2 antibody binding to an antigen-coated biosensor chip. Since RF-AN has been characterized by crystallography to bind to the CH2/CH3 interface of the IgG-Fc, and leukocyte FcγRIIa binds to a distinct site centred on the lower hinge, this inhibition is uncompetitive. Some inhibition (15%) of staphylococcal protein A binding to IC was also observed. As soluble FcγRIIa disrupts Fc:Fc interactions in IgG-ICs, we propose that this alteration of the IC also reduces the accessibility of Fc portions in the IC, resulting in the partial inhibition of ligands, particularly IgM RF, which bind Fc. We propose that the high concentrations of soluble FcγR found during inflammation can affect the properties of ICs and their interaction with the immune system.
AB - Soluble low-affinity receptors for IgG are known to inhibit immune complex (IC)-mediated inflammation, and expression by leukocytes is elevated in several inflammatory diseases. Immunoglobulin M (IgM) rheumatoid factors (RF), anti-Fc autoantibodies, are found in autoimmune diseases, such as rheumatoid arthritis (RA), as well as in normal immune responses. This study demonstrated that soluble FcγRIIa inhibits the interaction of rheumatoid factors with ICs. The recombinant soluble low-affinity FcγR, rsFcγRIIa, partially inhibited (30-70%) the rate of precipitation of soluble ICs by RF-positive RA sera. This required the normal interaction of FcγRIIa with Fc as the effect could be abrogated with the Fab fragment of the blocking mAb IV-3. Furthermore, rsFcγRIIa partially inhibited (40%) the binding of a monoclonal IgM RF (RF-AN) to an IC formed by IgG2 antibody binding to an antigen-coated biosensor chip. Since RF-AN has been characterized by crystallography to bind to the CH2/CH3 interface of the IgG-Fc, and leukocyte FcγRIIa binds to a distinct site centred on the lower hinge, this inhibition is uncompetitive. Some inhibition (15%) of staphylococcal protein A binding to IC was also observed. As soluble FcγRIIa disrupts Fc:Fc interactions in IgG-ICs, we propose that this alteration of the IC also reduces the accessibility of Fc portions in the IC, resulting in the partial inhibition of ligands, particularly IgM RF, which bind Fc. We propose that the high concentrations of soluble FcγR found during inflammation can affect the properties of ICs and their interaction with the immune system.
UR - http://www.scopus.com/inward/record.url?scp=0037505472&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2567.2003.01652.x
DO - 10.1046/j.1365-2567.2003.01652.x
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C2 - 12757620
AN - SCOPUS:0037505472
SN - 0019-2805
VL - 109
SP - 246
EP - 254
JO - Immunology
JF - Immunology
IS - 2
ER -