Soluble sustained release gene delivery system

Gilad Bachrach, Michael Friedman, Gladis Gilinski, Doron Steinberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Delivery of genetic substance to target cells remains an obstacle for efficient utilization of gene therapy approaches. In this study, we describe a formulation of methacrylate acid copolymer carrier of DNA, in which the release rate of the gene can be controlled by pH. Plasmid release was coupled with the polymer's dissolution, which was accelerated in alkali conditions. The released plasmid was intact and bioactive, although alteration from closed circular supercoil to relaxed conformation was observed. Confocal laser scanning microscopy detected the plasmid DNA along the central layers of the polymeric film. Gene delivery systems controlled by the dissolution of the polymeric films offer flexibility in quantity and size of the incorporated DNA, and therefore could have a potential for in vivo use.

Original languageAmerican English
Pages (from-to)811-814
Number of pages4
JournalJournal of Biomedical Materials Research - Part A
Volume77
Issue number4
DOIs
StatePublished - 15 Jun 2006
Externally publishedYes

Keywords

  • Gene delivery
  • Methacrylic acid copolymer
  • Sustained release

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