TY - JOUR
T1 - Some characteristics of the Mg-ATPase of isolated red beet vacuoles
AU - Admon, Arie
AU - Jacoby, Benjamin
AU - Goldschmidt, Eliezer E.
PY - 1981/7
Y1 - 1981/7
N2 - Mg-ATPase activity was demonstrated in isolated beetroot vacuoles. Phosphatase and Mg-ATPase activities were distinguished by inhibition of the phosphatase with molybdate. Phosphatase activity was found to be confined to the vacuolar sap, while ATPase activity was found in both the sap and the tonoplast membrane pellet. The Mg-ATPase was inhibited by N,N′-dicyclohexylcarbodiimide (DCCD) but not by ouabain and oligomycin. Mg-ATPase activity was enhanced by chlorides of various monovalent organic and inorganic cations. The pH optimum of Mg-ATPase activity was 7.0-7.5.
AB - Mg-ATPase activity was demonstrated in isolated beetroot vacuoles. Phosphatase and Mg-ATPase activities were distinguished by inhibition of the phosphatase with molybdate. Phosphatase activity was found to be confined to the vacuolar sap, while ATPase activity was found in both the sap and the tonoplast membrane pellet. The Mg-ATPase was inhibited by N,N′-dicyclohexylcarbodiimide (DCCD) but not by ouabain and oligomycin. Mg-ATPase activity was enhanced by chlorides of various monovalent organic and inorganic cations. The pH optimum of Mg-ATPase activity was 7.0-7.5.
UR - http://www.scopus.com/inward/record.url?scp=49049145793&partnerID=8YFLogxK
U2 - 10.1016/0304-4211(81)90285-6
DO - 10.1016/0304-4211(81)90285-6
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:49049145793
SN - 0304-4211
VL - 22
SP - 89
EP - 96
JO - Plant Science Letters
JF - Plant Science Letters
IS - 1
ER -