Some characteristics of the Mg-ATPase of isolated red beet vacuoles

Arie Admon; Benjamin Jacoby; Eliezer E. Goldschmidt

doi:10.1016/0304-4211(81)90285-6

Some characteristics of the Mg-ATPase of isolated red beet vacuoles

Arie Admon^*
, Benjamin Jacoby
, Eliezer E. Goldschmidt

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Mg-ATPase activity was demonstrated in isolated beetroot vacuoles. Phosphatase and Mg-ATPase activities were distinguished by inhibition of the phosphatase with molybdate. Phosphatase activity was found to be confined to the vacuolar sap, while ATPase activity was found in both the sap and the tonoplast membrane pellet. The Mg-ATPase was inhibited by N,N′-dicyclohexylcarbodiimide (DCCD) but not by ouabain and oligomycin. Mg-ATPase activity was enhanced by chlorides of various monovalent organic and inorganic cations. The pH optimum of Mg-ATPase activity was 7.0-7.5.

Original language	English
Pages (from-to)	89-96
Number of pages	8
Journal	Plant Science Letters
Volume	22
Issue number	1
DOIs	https://doi.org/10.1016/0304-4211(81)90285-6
State	Published - Jul 1981

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title = "Some characteristics of the Mg-ATPase of isolated red beet vacuoles",

abstract = "Mg-ATPase activity was demonstrated in isolated beetroot vacuoles. Phosphatase and Mg-ATPase activities were distinguished by inhibition of the phosphatase with molybdate. Phosphatase activity was found to be confined to the vacuolar sap, while ATPase activity was found in both the sap and the tonoplast membrane pellet. The Mg-ATPase was inhibited by N,N′-dicyclohexylcarbodiimide (DCCD) but not by ouabain and oligomycin. Mg-ATPase activity was enhanced by chlorides of various monovalent organic and inorganic cations. The pH optimum of Mg-ATPase activity was 7.0-7.5.",

author = "Arie Admon and Benjamin Jacoby and Goldschmidt, \{Eliezer E.\}",

year = "1981",

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doi = "10.1016/0304-4211(81)90285-6",

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AU - Jacoby, Benjamin

AU - Goldschmidt, Eliezer E.

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N2 - Mg-ATPase activity was demonstrated in isolated beetroot vacuoles. Phosphatase and Mg-ATPase activities were distinguished by inhibition of the phosphatase with molybdate. Phosphatase activity was found to be confined to the vacuolar sap, while ATPase activity was found in both the sap and the tonoplast membrane pellet. The Mg-ATPase was inhibited by N,N′-dicyclohexylcarbodiimide (DCCD) but not by ouabain and oligomycin. Mg-ATPase activity was enhanced by chlorides of various monovalent organic and inorganic cations. The pH optimum of Mg-ATPase activity was 7.0-7.5.

AB - Mg-ATPase activity was demonstrated in isolated beetroot vacuoles. Phosphatase and Mg-ATPase activities were distinguished by inhibition of the phosphatase with molybdate. Phosphatase activity was found to be confined to the vacuolar sap, while ATPase activity was found in both the sap and the tonoplast membrane pellet. The Mg-ATPase was inhibited by N,N′-dicyclohexylcarbodiimide (DCCD) but not by ouabain and oligomycin. Mg-ATPase activity was enhanced by chlorides of various monovalent organic and inorganic cations. The pH optimum of Mg-ATPase activity was 7.0-7.5.

UR - https://www.scopus.com/pages/publications/49049145793

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JF - Plant Science Letters

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Some characteristics of the Mg-ATPase of isolated red beet vacuoles

Abstract

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