Soybean Isoflavones. Characterization, Determination, and Antifungal Activity

Michael Naim, Benjamin Gestetner, Shmuel Zilkah, Yehudith Birk, Aron Bondi

Research output: Contribution to journalArticlepeer-review

195 Scopus citations

Abstract

The isolation and separation of defatted soybean meal isoflavone glycosides by chelation with aluminum oxide and subsequent chromatography on polyamide columns have been described. A procedure for the quantitative determination via gas chromatography of isoflavones in soybean meal was elaborated. The resulting analytical data showed that the content of isoflavones in soybeans amounts to 0.25% and that 99% of the isoflavones are present as glycosides: 64% genistin, 23% daidzin, and 13% glycitein 7-O-β-glycoside. Soybean oil does not contain isoflavones. The growth depression of Trichoderma lignorum, Rhizoctonia solani, Fusarium oxysporum, Pythium spp, Rhizopus spp, and Sclerotium rolfsii exerted by soybean isoflavones is due mainly to the presence of free isoflavones in the growth medium, while the activity of the glycosides is in most instances negligible.

Original languageEnglish
Pages (from-to)806-810
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume22
Issue number5
DOIs
StatePublished - 1 Sep 1974

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