Spacer effect on the synthesis of oligodeoxynucleotides by the phosphite method

J. Katzhendler; S. Cohen; M. Weisz; I. Ringel; R. D. Camerini-Oterio; J. Deutsch

doi:10.1016/0167-6989(87)90087-0

Spacer effect on the synthesis of oligodeoxynucleotides by the phosphite method

J. Katzhendler^*
, S. Cohen
, M. Weisz
, I. Ringel
, R. D. Camerini-Oterio
, J. Deutsch

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Nineteen oligonucleotides of 15-27 bases long were synthesized on aminoalkyl spacers of various chain lengths, SiO(CH₂)₃NHCONH(CH₂)_nNHCO(CH₂)_mNH₂, linked to Fractosil silica gel supports (F6, F12, F15, F18, F21, F24, F32, F38), to Porasil (P15) silica gel support, and to modified controlled pore glass (CPG17, CPG24). The purity and the homogenity of the end product in the reaction mixture was quantitatively determined from the autoradiograph pattern of bands on polyacrylamide sizing gel. The data indicated that the most pure products are obtained either on the shortest spacer, F6, or on the 24, 32, or 38 atom length spacers. Spacer lengths of 15, 18 and 21 atoms gave very poor results, with large amounts of impurities. The results are interpreted on the basis of conformational changes in the spacer alkyl chain as a function of its length.

Original language	English
Pages (from-to)	175-187
Number of pages	13
Journal	Reactive Polymers, Ion Exchangers, Sorbents
Volume	6
Issue number	2-3
DOIs	https://doi.org/10.1016/0167-6989(87)90087-0
State	Published - Oct 1987

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title = "Spacer effect on the synthesis of oligodeoxynucleotides by the phosphite method",

abstract = "Nineteen oligonucleotides of 15-27 bases long were synthesized on aminoalkyl spacers of various chain lengths, SiO(CH2)3NHCONH(CH2)nNHCO(CH2)mNH2, linked to Fractosil silica gel supports (F6, F12, F15, F18, F21, F24, F32, F38), to Porasil (P15) silica gel support, and to modified controlled pore glass (CPG17, CPG24). The purity and the homogenity of the end product in the reaction mixture was quantitatively determined from the autoradiograph pattern of bands on polyacrylamide sizing gel. The data indicated that the most pure products are obtained either on the shortest spacer, F6, or on the 24, 32, or 38 atom length spacers. Spacer lengths of 15, 18 and 21 atoms gave very poor results, with large amounts of impurities. The results are interpreted on the basis of conformational changes in the spacer alkyl chain as a function of its length.",

author = "J. Katzhendler and S. Cohen and M. Weisz and I. Ringel and Camerini-Oterio, \{R. D.\} and J. Deutsch",

year = "1987",

month = oct,

doi = "10.1016/0167-6989(87)90087-0",

language = "אנגלית",

volume = "6",

pages = "175--187",

journal = "Reactive Polymers, Ion Exchangers, Sorbents",

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TY - JOUR

T1 - Spacer effect on the synthesis of oligodeoxynucleotides by the phosphite method

AU - Katzhendler, J.

AU - Cohen, S.

AU - Weisz, M.

AU - Ringel, I.

AU - Camerini-Oterio, R. D.

AU - Deutsch, J.

PY - 1987/10

Y1 - 1987/10

N2 - Nineteen oligonucleotides of 15-27 bases long were synthesized on aminoalkyl spacers of various chain lengths, SiO(CH2)3NHCONH(CH2)nNHCO(CH2)mNH2, linked to Fractosil silica gel supports (F6, F12, F15, F18, F21, F24, F32, F38), to Porasil (P15) silica gel support, and to modified controlled pore glass (CPG17, CPG24). The purity and the homogenity of the end product in the reaction mixture was quantitatively determined from the autoradiograph pattern of bands on polyacrylamide sizing gel. The data indicated that the most pure products are obtained either on the shortest spacer, F6, or on the 24, 32, or 38 atom length spacers. Spacer lengths of 15, 18 and 21 atoms gave very poor results, with large amounts of impurities. The results are interpreted on the basis of conformational changes in the spacer alkyl chain as a function of its length.

AB - Nineteen oligonucleotides of 15-27 bases long were synthesized on aminoalkyl spacers of various chain lengths, SiO(CH2)3NHCONH(CH2)nNHCO(CH2)mNH2, linked to Fractosil silica gel supports (F6, F12, F15, F18, F21, F24, F32, F38), to Porasil (P15) silica gel support, and to modified controlled pore glass (CPG17, CPG24). The purity and the homogenity of the end product in the reaction mixture was quantitatively determined from the autoradiograph pattern of bands on polyacrylamide sizing gel. The data indicated that the most pure products are obtained either on the shortest spacer, F6, or on the 24, 32, or 38 atom length spacers. Spacer lengths of 15, 18 and 21 atoms gave very poor results, with large amounts of impurities. The results are interpreted on the basis of conformational changes in the spacer alkyl chain as a function of its length.

UR - https://www.scopus.com/pages/publications/0022797655

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DO - 10.1016/0167-6989(87)90087-0

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SN - 0167-6989

VL - 6

SP - 175

EP - 187

JO - Reactive Polymers, Ion Exchangers, Sorbents

JF - Reactive Polymers, Ion Exchangers, Sorbents

IS - 2-3

ER -

Spacer effect on the synthesis of oligodeoxynucleotides by the phosphite method

Abstract

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