SPATA18, a spermatogenesis-associated gene, is a novel transcriptional target of p53 and p63

Chamutal Bornstein, Ran Brosh*, Alina Molchadsky, Shalom Madar, Ira Kogan-Sakin, Ido Goldstein, Deepavali Chakravarti, Elsa R. Flores, Naomi Goldfinger, Rachel Sarig, Varda Rotter

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The transcription factor p53 functions not only to suppress tumorigenesis but also to maintain normal development and homeostasis. Although p53 was implicated in different aspects of fertility, including spermatogenesis and implantation, the mechanism underlying p53 involvement in spermatogenesis is poorly resolved. In this study we describe the identification of a spermatogenesis-associated gene, SPATA18, as a novel p53 transcriptional target and show that SPATA18 transcription is induced by p53 in a variety of cell types of both human and mouse origin. p53 binds a consensus DNA motif that resides within the first intron of SPATA18. We describe the spatiotemporal expression patterns of SPATA18 in mouse seminiferous tubules and suggest that SPATA18 transcription is regulated in vivo by p53. We also demonstrate the induction of SPATA18 by p63 and suggest that p63 can compensate for the loss of p53 activity in vivo. Our data not only enrich the known collection of p53 targets but may also provide insights on spermatogenesis defects that are associated with p53 deficiency.

Original languageAmerican English
Pages (from-to)1679-1689
Number of pages11
JournalMolecular and Cellular Biology
Volume31
Issue number8
DOIs
StatePublished - Apr 2011
Externally publishedYes

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