Abstract
The human RUNX3/AML2 gene belongs to the 'runt domain' family of transcription factors that act as gene expression regulators in major developmental pathways. Here, we describe the expression pattern of Runx3 during mouse embryogenesis compared to the expression pattern of Runx1. E10.5 and E14.5-E16.5 embryos were analyzed using both immunohistochemistry and β-galactosidase activity of targeted Runx3 and Runx1 loci. We found that Runx3 expression overlapped with that of Runx1 in the hematopoietic system, whereas in sensory ganglia, epidermal appendages, and developing skeletal elements, their expression was confined to different compartments. These data provide new insights into the function of Runx3 and Runx1 in organogenesis and support the possibility that cross-regulation between them plays a role in embryogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 413-417 |
| Number of pages | 5 |
| Journal | Mechanisms of Development |
| Volume | 109 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2001 |
Bibliographical note
Funding Information:We thank Dorit Nathan, Judith Chermesh and Shoshana Grossfeld for excellent technical assistance. This work was supported by grants from the Commission of the EU, the Israel Science Foundation and Shapell Family Biomedical Research Foundation at the Weizmann Institute.
Keywords
- AML1 and AML2
- Cartilage
- Dorsal root ganglia
- Epidermal appendages
- Epithelial-mesenchymal interactions
- Mouse embryogenesis
- Organogenesis
- Runt transcription factors
- Spatio-temporal expression
- Tissue-specific expression