TY - JOUR
T1 - Species-specific detection of Leishmania in sandflies using an enzyme-linked immunosorbent assay
AU - Adini, Irit
AU - Jacobson, Raymond L.
AU - Kasap, Mulkiye
AU - Schlein, Yosef
AU - Jaffe, Charles L.
PY - 1998
Y1 - 1998
N2 - A direct enzyme-linked immunosorbent assay (ELISA) using species-specific monoclonal antibodies was developed for the detection and identification of Leishmania in sandflies. A titration of mock-infected Phlebotomus papatasi showed that fewer than 2000 L. major promastigotes could be detected. The percentage of infected P. papatasi collected in the field, as determined by dissection, was compared to that revealed by the ELISA. Both methods gave similar results, irrespective of whether the flies were caught by sticky papers or light-traps. The percentage of infected flies determined by either method was also similar in experimentally infected colony reared sandflies. The ELISA can be carried out using multiple species-specific antibodies, and is as accurate as identification of infected sandflies by microscopical examination. The technique should be useful for identifying sandfly species involved in transmitting different species of Leishmania, and for rapid assessment of leishmanial infection rates in endemic regions.
AB - A direct enzyme-linked immunosorbent assay (ELISA) using species-specific monoclonal antibodies was developed for the detection and identification of Leishmania in sandflies. A titration of mock-infected Phlebotomus papatasi showed that fewer than 2000 L. major promastigotes could be detected. The percentage of infected P. papatasi collected in the field, as determined by dissection, was compared to that revealed by the ELISA. Both methods gave similar results, irrespective of whether the flies were caught by sticky papers or light-traps. The percentage of infected flies determined by either method was also similar in experimentally infected colony reared sandflies. The ELISA can be carried out using multiple species-specific antibodies, and is as accurate as identification of infected sandflies by microscopical examination. The technique should be useful for identifying sandfly species involved in transmitting different species of Leishmania, and for rapid assessment of leishmanial infection rates in endemic regions.
KW - Detection
KW - Enzyme-linked immunosorbent assay
KW - Infection
KW - Israel
KW - Leishmania major
KW - Leishmaniasis
KW - Phlebotomus papatasi
UR - http://www.scopus.com/inward/record.url?scp=0031883808&partnerID=8YFLogxK
U2 - 10.1016/S0035-9203(98)90946-4
DO - 10.1016/S0035-9203(98)90946-4
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C2 - 9692146
AN - SCOPUS:0031883808
SN - 0035-9203
VL - 92
SP - 35
EP - 37
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
IS - 1
ER -