Specific and efficient adsorption of phosphorothioated DNA on Au-based surfaces and electrodes

Jamal Ghabboun; Mukhles Sowwan; Hezy Cohen; Tatiana Molotsky; Natalia Borovok; Benjamin Dwir; Eli Kapon; Alexander Kotlyar; Danny Porath

doi:10.1063/1.2800383

Specific and efficient adsorption of phosphorothioated DNA on Au-based surfaces and electrodes

Jamal Ghabboun, Mukhles Sowwan, Hezy Cohen, Tatiana Molotsky, Natalia Borovok, Benjamin Dwir, Eli Kapon, Alexander Kotlyar^*, Danny Porath

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Efficient attachment of DNA to metal surfaces or electrodes is essential for charge-transport measurements, scanning tunneling microscopy, and for devices and sensors. To optimize DNA deposition on Au-based surfaces and electrodes, we synthesized DNA with phosphorothioate (PT) groups attached to the G strand of poly(deoxyguanine)-poly(deoxycytosine) [poly(dG)-poly(dC)]. This procedure strongly improves the DNA anchoring to Au-based surfaces by sulfur-gold interaction. Much higher molecular surface density on Au substrates was observed for PT poly(dG)-poly(dC) compared to "bare" molecules. Deposition of PT poly(dG)-poly(dC) on Au-based electrodes, followed by thorough washing, showed that they specifically attach to the electrodes and are not spread on the surrounding Si O2 surface.

Original language	English
Article number	173101
Journal	Applied Physics Letters
Volume	91
Issue number	17
DOIs	https://doi.org/10.1063/1.2800383
State	Published - 2007

Bibliographical note

Funding Information:
We thank Igor Brodsky and Moti Zimmermann for technical assistance. We acknowledge financial support from the European Union through IST Future and Emerging Technologies Projects: Nos. IST-2001-38951 (“DNA-Based Nanowires”) and FP6-029192 (“DNA-Based Nanodevices”) and of the French Ministry of External Affairs, the Lazard Frères Foundation and the Israeli-Palestinian Science Organization(IPSO).

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title = "Specific and efficient adsorption of phosphorothioated DNA on Au-based surfaces and electrodes",

abstract = "Efficient attachment of DNA to metal surfaces or electrodes is essential for charge-transport measurements, scanning tunneling microscopy, and for devices and sensors. To optimize DNA deposition on Au-based surfaces and electrodes, we synthesized DNA with phosphorothioate (PT) groups attached to the G strand of poly(deoxyguanine)-poly(deoxycytosine) [poly(dG)-poly(dC)]. This procedure strongly improves the DNA anchoring to Au-based surfaces by sulfur-gold interaction. Much higher molecular surface density on Au substrates was observed for PT poly(dG)-poly(dC) compared to {"}bare{"} molecules. Deposition of PT poly(dG)-poly(dC) on Au-based electrodes, followed by thorough washing, showed that they specifically attach to the electrodes and are not spread on the surrounding Si O2 surface.",

author = "Jamal Ghabboun and Mukhles Sowwan and Hezy Cohen and Tatiana Molotsky and Natalia Borovok and Benjamin Dwir and Eli Kapon and Alexander Kotlyar and Danny Porath",

note = "Funding Information: We thank Igor Brodsky and Moti Zimmermann for technical assistance. We acknowledge financial support from the European Union through IST Future and Emerging Technologies Projects: Nos. IST-2001-38951 (“DNA-Based Nanowires”) and FP6-029192 (“DNA-Based Nanodevices”) and of the French Ministry of External Affairs, the Lazard Fr{\`e}res Foundation and the Israeli-Palestinian Science Organization(IPSO).",

year = "2007",

doi = "10.1063/1.2800383",

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volume = "91",

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AU - Ghabboun, Jamal

AU - Sowwan, Mukhles

AU - Cohen, Hezy

AU - Molotsky, Tatiana

AU - Borovok, Natalia

AU - Dwir, Benjamin

AU - Kapon, Eli

AU - Kotlyar, Alexander

AU - Porath, Danny

N1 - Funding Information: We thank Igor Brodsky and Moti Zimmermann for technical assistance. We acknowledge financial support from the European Union through IST Future and Emerging Technologies Projects: Nos. IST-2001-38951 (“DNA-Based Nanowires”) and FP6-029192 (“DNA-Based Nanodevices”) and of the French Ministry of External Affairs, the Lazard Frères Foundation and the Israeli-Palestinian Science Organization(IPSO).

PY - 2007

Y1 - 2007

N2 - Efficient attachment of DNA to metal surfaces or electrodes is essential for charge-transport measurements, scanning tunneling microscopy, and for devices and sensors. To optimize DNA deposition on Au-based surfaces and electrodes, we synthesized DNA with phosphorothioate (PT) groups attached to the G strand of poly(deoxyguanine)-poly(deoxycytosine) [poly(dG)-poly(dC)]. This procedure strongly improves the DNA anchoring to Au-based surfaces by sulfur-gold interaction. Much higher molecular surface density on Au substrates was observed for PT poly(dG)-poly(dC) compared to "bare" molecules. Deposition of PT poly(dG)-poly(dC) on Au-based electrodes, followed by thorough washing, showed that they specifically attach to the electrodes and are not spread on the surrounding Si O2 surface.

AB - Efficient attachment of DNA to metal surfaces or electrodes is essential for charge-transport measurements, scanning tunneling microscopy, and for devices and sensors. To optimize DNA deposition on Au-based surfaces and electrodes, we synthesized DNA with phosphorothioate (PT) groups attached to the G strand of poly(deoxyguanine)-poly(deoxycytosine) [poly(dG)-poly(dC)]. This procedure strongly improves the DNA anchoring to Au-based surfaces by sulfur-gold interaction. Much higher molecular surface density on Au substrates was observed for PT poly(dG)-poly(dC) compared to "bare" molecules. Deposition of PT poly(dG)-poly(dC) on Au-based electrodes, followed by thorough washing, showed that they specifically attach to the electrodes and are not spread on the surrounding Si O2 surface.

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Specific and efficient adsorption of phosphorothioated DNA on Au-based surfaces and electrodes

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