Specific and efficient adsorption of phosphorothioated DNA on Au-based surfaces and electrodes

Jamal Ghabboun, Mukhles Sowwan, Hezy Cohen, Tatiana Molotsky, Natalia Borovok, Benjamin Dwir, Eli Kapon, Alexander Kotlyar*, Danny Porath

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Efficient attachment of DNA to metal surfaces or electrodes is essential for charge-transport measurements, scanning tunneling microscopy, and for devices and sensors. To optimize DNA deposition on Au-based surfaces and electrodes, we synthesized DNA with phosphorothioate (PT) groups attached to the G strand of poly(deoxyguanine)-poly(deoxycytosine) [poly(dG)-poly(dC)]. This procedure strongly improves the DNA anchoring to Au-based surfaces by sulfur-gold interaction. Much higher molecular surface density on Au substrates was observed for PT poly(dG)-poly(dC) compared to "bare" molecules. Deposition of PT poly(dG)-poly(dC) on Au-based electrodes, followed by thorough washing, showed that they specifically attach to the electrodes and are not spread on the surrounding Si O2 surface.

Original languageAmerican English
Article number173101
JournalApplied Physics Letters
Volume91
Issue number17
DOIs
StatePublished - 2007

Bibliographical note

Funding Information:
We thank Igor Brodsky and Moti Zimmermann for technical assistance. We acknowledge financial support from the European Union through IST Future and Emerging Technologies Projects: Nos. IST-2001-38951 (“DNA-Based Nanowires”) and FP6-029192 (“DNA-Based Nanodevices”) and of the French Ministry of External Affairs, the Lazard Frères Foundation and the Israeli-Palestinian Science Organization(IPSO).

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