Specificity of Ca2+ and Mg2+ binding to phosphatidylserine vesicles and resultant phase changes of bilayer membrane structure

Carolyn Newton*, Walter Pangborn, Shlomo Nir, Demetrios Papahadjopoulos

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

192 Scopus citations

Abstract

Differences in the interaction of Ca2+ and Mg2+ with phosphatidylserine vesicles were revealed by binding studies, differential scanning calorimetry and X-ray diffraction. The two cations produced structurally different complexes with phosphatidylserine as evidenced by phase transition characteristics, lamellar spacings, and hydrocarbon packing. Ca2+ effectively competes with Mg2+ for phosphatidylserine binding sites. Analysis of the binding data showed that Ca2+ had a ten-fold greater intrinsic binding constant for phosphatidylserine.

Original languageEnglish
Pages (from-to)281-287
Number of pages7
JournalBiochimica et Biophysica Acta - Biomembranes
Volume506
Issue number2
DOIs
StatePublished - 19 Jan 1978
Externally publishedYes

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