Specificity of Ca2+ and Mg2+ binding to phosphatidylserine vesicles and resultant phase changes of bilayer membrane structure

Carolyn Newton; Walter Pangborn; Shlomo Nir; Demetrios Papahadjopoulos

doi:10.1016/0005-2736(78)90398-X

Specificity of Ca²⁺ and Mg²⁺ binding to phosphatidylserine vesicles and resultant phase changes of bilayer membrane structure

Carolyn Newton^*
, Walter Pangborn
, Shlomo Nir
, Demetrios Papahadjopoulos

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

194 Scopus citations

Abstract

Differences in the interaction of Ca²⁺ and Mg²⁺ with phosphatidylserine vesicles were revealed by binding studies, differential scanning calorimetry and X-ray diffraction. The two cations produced structurally different complexes with phosphatidylserine as evidenced by phase transition characteristics, lamellar spacings, and hydrocarbon packing. Ca²⁺ effectively competes with Mg²⁺ for phosphatidylserine binding sites. Analysis of the binding data showed that Ca²⁺ had a ten-fold greater intrinsic binding constant for phosphatidylserine.

Original language	English
Pages (from-to)	281-287
Number of pages	7
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	506
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(78)90398-X
State	Published - 19 Jan 1978
Externally published	Yes

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title = "Specificity of Ca2+ and Mg2+ binding to phosphatidylserine vesicles and resultant phase changes of bilayer membrane structure",

abstract = "Differences in the interaction of Ca2+ and Mg2+ with phosphatidylserine vesicles were revealed by binding studies, differential scanning calorimetry and X-ray diffraction. The two cations produced structurally different complexes with phosphatidylserine as evidenced by phase transition characteristics, lamellar spacings, and hydrocarbon packing. Ca2+ effectively competes with Mg2+ for phosphatidylserine binding sites. Analysis of the binding data showed that Ca2+ had a ten-fold greater intrinsic binding constant for phosphatidylserine.",

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AU - Newton, Carolyn

AU - Pangborn, Walter

AU - Nir, Shlomo

AU - Papahadjopoulos, Demetrios

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N2 - Differences in the interaction of Ca2+ and Mg2+ with phosphatidylserine vesicles were revealed by binding studies, differential scanning calorimetry and X-ray diffraction. The two cations produced structurally different complexes with phosphatidylserine as evidenced by phase transition characteristics, lamellar spacings, and hydrocarbon packing. Ca2+ effectively competes with Mg2+ for phosphatidylserine binding sites. Analysis of the binding data showed that Ca2+ had a ten-fold greater intrinsic binding constant for phosphatidylserine.

AB - Differences in the interaction of Ca2+ and Mg2+ with phosphatidylserine vesicles were revealed by binding studies, differential scanning calorimetry and X-ray diffraction. The two cations produced structurally different complexes with phosphatidylserine as evidenced by phase transition characteristics, lamellar spacings, and hydrocarbon packing. Ca2+ effectively competes with Mg2+ for phosphatidylserine binding sites. Analysis of the binding data showed that Ca2+ had a ten-fold greater intrinsic binding constant for phosphatidylserine.

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Specificity of Ca²⁺ and Mg²⁺ binding to phosphatidylserine vesicles and resultant phase changes of bilayer membrane structure

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