Spinal lumbar di2 interneurons contribute to stability of bipedal stepping

Baruch Haimson; Yoav Hadas; Nimrod Bernat; Artur Kania; Monica Daley; Yuval Cinnamon; Aharon Lev-Tov; Avihu Klar

doi:10.7554/eLife.62001

Spinal lumbar di2 interneurons contribute to stability of bipedal stepping

Baruch Haimson, Yoav Hadas, Nimrod Bernat, Artur Kania, Monica Daley, Yuval Cinnamon, Aharon Lev-Tov, Avihu Klar^*

^*Corresponding author for this work

Department of Medical Neurobiology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Peripheral and intraspinal feedback is required to shape and update the output of spinal networks that execute motor behavior. We report that lumbar dI2 spinal interneurons in chicks receive synaptic input from afferents and premotor neurons. These interneurons innervate contralateral premotor networks in the lumbar and brachial spinal cord, and their ascending projections innervate the cerebellum. These findings suggest that dI2 neurons function as interneurons in local lumbar circuits, are involved in lumbo-brachial coupling, and that part of them deliver peripheral and intraspinal feedback to the cerebellum. Silencing of dI2 neurons leads to destabilized stepping in P8 hatchlings, with occasional collapses, variable step profiles and a wide-base walking gait, suggesting that dI2 neurons may contribute to the stabilization of the bipedal gait.

Original language	American English
Journal	eLife
Volume	10
DOIs	https://doi.org/10.7554/eLife.62001
State	Published - Aug 2021

Bibliographical note

Funding Information:
to AK from the Israel Science Foundation (grant No. 1400/16), the USʹIsrael Binational

Funding Information:
Science Foundation (grant No. 2017/172) and the Avraham and Ida Baruch endowment fund.

Funding Information:
The authors thank Haya Falk for PRV purification; Alona Katzir, Cole Bendor, Mevaseret Avital, Sapir Shevah, Eitan Yisraeli, Ruth Segal, Fedaa Bazan and Eden Kimchi for technical assistance; Nadav Yayon for assistance with the light sheet microscopy; and Michael O?Donovan for comments on the manuscript. This work was supported by grants to AK from the Israel Science Foundation (grant No. 1400/16), the US?Israel Binational Science Foundation (grant No. 2017/172) and the Avraham and Ida Baruch endowment fund.

Publisher Copyright:
© 2021, eLife Sciences Publications Ltd. All rights reserved.

Keywords

Locomotion
Neuronal circuits
Spinal cord
Spinocerebellar tract

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10.7554/eLife.62001

Cite this

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title = "Spinal lumbar di2 interneurons contribute to stability of bipedal stepping",

abstract = "Peripheral and intraspinal feedback is required to shape and update the output of spinal networks that execute motor behavior. We report that lumbar dI2 spinal interneurons in chicks receive synaptic input from afferents and premotor neurons. These interneurons innervate contralateral premotor networks in the lumbar and brachial spinal cord, and their ascending projections innervate the cerebellum. These findings suggest that dI2 neurons function as interneurons in local lumbar circuits, are involved in lumbo-brachial coupling, and that part of them deliver peripheral and intraspinal feedback to the cerebellum. Silencing of dI2 neurons leads to destabilized stepping in P8 hatchlings, with occasional collapses, variable step profiles and a wide-base walking gait, suggesting that dI2 neurons may contribute to the stabilization of the bipedal gait.",

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author = "Baruch Haimson and Yoav Hadas and Nimrod Bernat and Artur Kania and Monica Daley and Yuval Cinnamon and Aharon Lev-Tov and Avihu Klar",

note = "Funding Information: to AK from the Israel Science Foundation (grant No. 1400/16), the USʹIsrael Binational Funding Information: Science Foundation (grant No. 2017/172) and the Avraham and Ida Baruch endowment fund. Funding Information: The authors thank Haya Falk for PRV purification; Alona Katzir, Cole Bendor, Mevaseret Avital, Sapir Shevah, Eitan Yisraeli, Ruth Segal, Fedaa Bazan and Eden Kimchi for technical assistance; Nadav Yayon for assistance with the light sheet microscopy; and Michael O?Donovan for comments on the manuscript. This work was supported by grants to AK from the Israel Science Foundation (grant No. 1400/16), the US?Israel Binational Science Foundation (grant No. 2017/172) and the Avraham and Ida Baruch endowment fund. Publisher Copyright: {\textcopyright} 2021, eLife Sciences Publications Ltd. All rights reserved.",

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AU - Lev-Tov, Aharon

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N2 - Peripheral and intraspinal feedback is required to shape and update the output of spinal networks that execute motor behavior. We report that lumbar dI2 spinal interneurons in chicks receive synaptic input from afferents and premotor neurons. These interneurons innervate contralateral premotor networks in the lumbar and brachial spinal cord, and their ascending projections innervate the cerebellum. These findings suggest that dI2 neurons function as interneurons in local lumbar circuits, are involved in lumbo-brachial coupling, and that part of them deliver peripheral and intraspinal feedback to the cerebellum. Silencing of dI2 neurons leads to destabilized stepping in P8 hatchlings, with occasional collapses, variable step profiles and a wide-base walking gait, suggesting that dI2 neurons may contribute to the stabilization of the bipedal gait.

AB - Peripheral and intraspinal feedback is required to shape and update the output of spinal networks that execute motor behavior. We report that lumbar dI2 spinal interneurons in chicks receive synaptic input from afferents and premotor neurons. These interneurons innervate contralateral premotor networks in the lumbar and brachial spinal cord, and their ascending projections innervate the cerebellum. These findings suggest that dI2 neurons function as interneurons in local lumbar circuits, are involved in lumbo-brachial coupling, and that part of them deliver peripheral and intraspinal feedback to the cerebellum. Silencing of dI2 neurons leads to destabilized stepping in P8 hatchlings, with occasional collapses, variable step profiles and a wide-base walking gait, suggesting that dI2 neurons may contribute to the stabilization of the bipedal gait.

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Spinal lumbar di2 interneurons contribute to stability of bipedal stepping

Abstract

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Keywords

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