Spreading and proliferation of cultured rat bone marrow stromal cells on the surface of bioactive glass ceramics

Aim. To study spreading profile, cytoskeleton structure organization, and proliferation of bone marrow stromal cells in the course of cultivation on the glass-crystalline material (GCM) surfaces, with different chemical composition and solubility. Methods. GCMs with different CaO: P₂O₅ ratios were used. The actins cytoskeleton in cells was visualized using fluorescent TRITC-conjugated phalloidin. Cell proliferation was studied using MTT test. Results. The cell cultivation on highly soluble B series GCM (Ca/P = 5 superficial ratio) led to appearance of fibroblast-like cells, the actin cytoskeleton filaments of which were uniformly distributed within cytoplasm. In this case, proliferation dynamics was similar to that] under cultivation on plastic. Ca/P ratio reduction on the surfaces of A and C series of GCMs to 1.4 ÷ 2.58 resulted in a decrease of spreading area and proliferation index (up to 2–3 times) relative to the control. Conclusions. Main factors, determining the cells interaction behavior with GCM are the materials solubility and Ca/P superficial ratio.