Stability of nano-sized permethrin in its colloidal state and its effect on the physiological and biochemical profile of Culex tritaeniorhynchus larvae

P. Mishra, A. P.B. Balaji, P. K. Dhal, R. S. Suresh Kumar, S. Magdassi, K. Margulis, B. K. Tyagi, A. Mukherjee, N. Chandrasekaran*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The occurrence of pesticidal pollution in the environment and the resistance in the mosquito species makes an urge for the safer and an effective pesticide. Permethrin, a poorly water-soluble pyrethroid pesticide, was formulated into a hydrodispersible nanopowder through rapid solvent evaporation of pesticide-loaded oil in water microemulsion. Stability studies confirmed that the nanopermethrin dispersion was stable in paddy field water for 5 days with the mean particle sizes of 175.3 ± 0.75 nm and zeta potential of -30.6 ± 0.62 mV. The instability rate of the nanopermethrin particles was greater in alkaline (pH 10) medium when compared with the neutral (pH 7) and acidic (pH 4) dispersion medium. The colloidal dispersion at 45°C was found to be less stable compared with the dispersions at 25 and 5°C. The 12- and 24-h lethal indices (LC50) for nanopermethrin were found to be 0.057 and 0.014 mg l-1, respectively. These results were corroborative with the severity of damages observed in the mosquito larvae manifested in epithelial cells and the evacuation of the midgut contents. Further, the results were substantiated by the decrease in cellular biomolecules and biomarker enzyme activity in nanopermethrin treated larvae when compared to bulk and control treatment.

Original languageAmerican English
Pages (from-to) 676–688
Number of pages13
JournalBulletin of Entomological Research
Volume107
Issue number5
DOIs
StatePublished - 1 Mar 2017

Bibliographical note

Publisher Copyright:
Copyright © Cambridge University Press 2017

Keywords

  • Culex tritaeniorhynchus
  • Japanese encephalitis
  • colloidal stability
  • nanopesticide
  • permethrin

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