Stability of Trichoderma harzianum amdS transformants in soil and rhizosphere

S. Pe'er*, Z. Barak, O. Yarden, I. Chet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Trichoderma harzianum was transformed with a plasmid p3SR2, carrying the dominant selectable marker amdS. The amdS transformants can be identified, from non-sterile soil. on Trichoderma-selective medium (TSM). containing acrylamide as the sole nitrogen source. The transformants were stable after at least 6 weeks in non-sterile soil in the greenhouse and after 14 months in sterile soil at 4°C. Soil inoculation resulted in effective colonization of the roots by the transformants. The advantages of these transformant fungi in the study of fungal ecology and population dynamics is discussed.

Original languageAmerican English
Pages (from-to)1043-1046
Number of pages4
JournalSoil Biology and Biochemistry
Volume23
Issue number11
DOIs
StatePublished - 1991

Bibliographical note

Funding Information:
Aclinoa,fedRfnr~nnrs_ur work was supported by a grant from the United States-Israel Binational Agricultural Research and ~evelopmcnt Fund (BARD) and a scholar- ship from the Val~hi-Pikovski fund. Our thanks are ex-tended to MS D. Rubin for technical assistance.

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