Stability of Trichoderma harzianum amdS transformants in soil and rhizosphere

S. Pe'er; Z. Barak; O. Yarden; I. Chet

doi:10.1016/0038-0717(91)90041-H

Stability of Trichoderma harzianum amdS transformants in soil and rhizosphere

S. Pe'er^*, Z. Barak, O. Yarden, I. Chet

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Trichoderma harzianum was transformed with a plasmid p3SR2, carrying the dominant selectable marker amdS. The amdS transformants can be identified, from non-sterile soil. on Trichoderma-selective medium (TSM). containing acrylamide as the sole nitrogen source. The transformants were stable after at least 6 weeks in non-sterile soil in the greenhouse and after 14 months in sterile soil at 4°C. Soil inoculation resulted in effective colonization of the roots by the transformants. The advantages of these transformant fungi in the study of fungal ecology and population dynamics is discussed.

Original language	English
Pages (from-to)	1043-1046
Number of pages	4
Journal	Soil Biology and Biochemistry
Volume	23
Issue number	11
DOIs	https://doi.org/10.1016/0038-0717(91)90041-H
State	Published - 1991

Bibliographical note

Funding Information:
Aclinoa,fedRfnr~nnrs_ur work was supported by a grant from the United States-Israel Binational Agricultural Research and ~evelopmcnt Fund (BARD) and a scholar- ship from the Val~hi-Pikovski fund. Our thanks are ex-tended to MS D. Rubin for technical assistance.

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title = "Stability of Trichoderma harzianum amdS transformants in soil and rhizosphere",

abstract = "Trichoderma harzianum was transformed with a plasmid p3SR2, carrying the dominant selectable marker amdS. The amdS transformants can be identified, from non-sterile soil. on Trichoderma-selective medium (TSM). containing acrylamide as the sole nitrogen source. The transformants were stable after at least 6 weeks in non-sterile soil in the greenhouse and after 14 months in sterile soil at 4°C. Soil inoculation resulted in effective colonization of the roots by the transformants. The advantages of these transformant fungi in the study of fungal ecology and population dynamics is discussed.",

author = "S. Pe'er and Z. Barak and O. Yarden and I. Chet",

note = "Funding Information: Aclinoa,fedRfnr~nnrs_ur work was supported by a grant from the United States-Israel Binational Agricultural Research and ~evelopmcnt Fund (BARD) and a scholar- ship from the Val~hi-Pikovski fund. Our thanks are ex-tended to MS D. Rubin for technical assistance.",

year = "1991",

doi = "10.1016/0038-0717(91)90041-H",

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AU - Yarden, O.

AU - Chet, I.

N1 - Funding Information: Aclinoa,fedRfnr~nnrs_ur work was supported by a grant from the United States-Israel Binational Agricultural Research and ~evelopmcnt Fund (BARD) and a scholar- ship from the Val~hi-Pikovski fund. Our thanks are ex-tended to MS D. Rubin for technical assistance.

PY - 1991

Y1 - 1991

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AB - Trichoderma harzianum was transformed with a plasmid p3SR2, carrying the dominant selectable marker amdS. The amdS transformants can be identified, from non-sterile soil. on Trichoderma-selective medium (TSM). containing acrylamide as the sole nitrogen source. The transformants were stable after at least 6 weeks in non-sterile soil in the greenhouse and after 14 months in sterile soil at 4°C. Soil inoculation resulted in effective colonization of the roots by the transformants. The advantages of these transformant fungi in the study of fungal ecology and population dynamics is discussed.

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JO - Soil Biology and Biochemistry

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ER -

Stability of Trichoderma harzianum amdS transformants in soil and rhizosphere

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