Stamens and gibberellic acid in the regulation of flavonoid gene expression in the corolla of Petunia hybrida

David Weiss, Arjen J. Van Tunen, Abraham H. Halevy*, Joseph N.M. Mol, Anton G.M. Gerats

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Stamen removal at an early stage of flower development inhibits anthocyanin synthesis and chalcone flavanon isomerase (CHI) enzyme activity in corollas of Petunia hybrida. The inhibition can be overcome by gibberellic acid (GA3) application. Gibberellin also induces anthocyanin synthesis in detached, young green corollas, grown in vitro in a sucrose medium and promotes CHI enzyme activity. Western blot analysis indicates an increase in chalcone synthase (CHS) and CHI protein levels following GA3 treatment in both the in vivo and the in vitro systems. Northern blot analysis shows a higher level of steady-state mRNAs for CHS and CHI 24 hours after GA3 application. In corollas from a transgenic plant containing a β-glucuronidase gene driven by a CHI promoter, a sixfold increase of β-glucuronidase activity was measured following GA3 application. The mode of action of stamens and GA3 control over flavonoid gene expression is discussed.

Original languageAmerican English
Pages (from-to)511-515
Number of pages5
JournalPlant Physiology
Volume94
Issue number2
DOIs
StatePublished - Oct 1990

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