Starch in guard cells functions in osmoregulation during stomatal movements. Starch metabolism is controlled by the circadian clock. We investigated the role of starch metabolism in stomatal responses to CO2 under different photoperiodic conditions. Guard cell starch levels correlate with low/high [CO2] exposure. Starch biosynthesis-deficient AGPase (ADG1) mutants but, unexpectedly, not the starch degradation-deficient BAM1, BAM3, and SEX1 mutants alone, are rate-limiting for stomatal conductance responses to [CO2]-shifts. Interestingly, AGPase is rate-limiting solely under short- but not long-day conditions. These findings suggest a model of enhanced AGPase activity in guard cells under short days such that starch biosynthesis becomes rate-limiting for CO2-induced stomatal closing.
Bibliographical noteFunding Information:
This work was funded by a grant from the National Science Foundation (grant number MCB-1616236) to JIS and was in part supported by postdoctoral fellowships from the US-Israel Binational Agricultural Research and Development Fund (BARD F1-446-11) to TA-S, by the UCSD Frontiers of Innovation Scholars Program (FISP) to TA-S and by the Israel Ministry of Aliyah and Integration.
© 2018 Federation of European Biochemical Societies
- gas exchange
- guard cell
- starch degradation
- starch synthesis