Starch biosynthesis by AGPase, but not starch degradation by BAM1/3 and SEX1, is rate-limiting for CO2-regulated stomatal movements under short-day conditions

Tamar Azoulay-Shemer; Nikki Schwankl; Ido Rog; Menachem Moshelion; Julian I. Schroeder

doi:10.1002/1873-3468.13198

Starch biosynthesis by AGPase, but not starch degradation by BAM1/3 and SEX1, is rate-limiting for CO₂-regulated stomatal movements under short-day conditions

Tamar Azoulay-Shemer, Nikki Schwankl, Ido Rog, Menachem Moshelion, Julian I. Schroeder^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Starch in guard cells functions in osmoregulation during stomatal movements. Starch metabolism is controlled by the circadian clock. We investigated the role of starch metabolism in stomatal responses to CO₂ under different photoperiodic conditions. Guard cell starch levels correlate with low/high [CO₂] exposure. Starch biosynthesis-deficient AGPase (ADG1) mutants but, unexpectedly, not the starch degradation-deficient BAM1, BAM3, and SEX1 mutants alone, are rate-limiting for stomatal conductance responses to [CO₂]-shifts. Interestingly, AGPase is rate-limiting solely under short- but not long-day conditions. These findings suggest a model of enhanced AGPase activity in guard cells under short days such that starch biosynthesis becomes rate-limiting for CO₂-induced stomatal closing.

Original language	American English
Pages (from-to)	2739-2759
Number of pages	21
Journal	FEBS Letters
Volume	592
Issue number	16
DOIs	https://doi.org/10.1002/1873-3468.13198
State	Published - Aug 2018

Bibliographical note

Funding Information:
This work was funded by a grant from the National Science Foundation (grant number MCB-1616236) to JIS and was in part supported by postdoctoral fellowships from the US-Israel Binational Agricultural Research and Development Fund (BARD F1-446-11) to TA-S, by the UCSD Frontiers of Innovation Scholars Program (FISP) to TA-S and by the Israel Ministry of Aliyah and Integration.

Publisher Copyright:
© 2018 Federation of European Biochemical Societies

Keywords

Arabidopsis
gas exchange
guard cell
photoperiod
starch degradation
starch synthesis

Access to Document

10.1002/1873-3468.13198

Cite this

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title = "Starch biosynthesis by AGPase, but not starch degradation by BAM1/3 and SEX1, is rate-limiting for CO2-regulated stomatal movements under short-day conditions",

abstract = "Starch in guard cells functions in osmoregulation during stomatal movements. Starch metabolism is controlled by the circadian clock. We investigated the role of starch metabolism in stomatal responses to CO2 under different photoperiodic conditions. Guard cell starch levels correlate with low/high [CO2] exposure. Starch biosynthesis-deficient AGPase (ADG1) mutants but, unexpectedly, not the starch degradation-deficient BAM1, BAM3, and SEX1 mutants alone, are rate-limiting for stomatal conductance responses to [CO2]-shifts. Interestingly, AGPase is rate-limiting solely under short- but not long-day conditions. These findings suggest a model of enhanced AGPase activity in guard cells under short days such that starch biosynthesis becomes rate-limiting for CO2-induced stomatal closing.",

keywords = "Arabidopsis, gas exchange, guard cell, photoperiod, starch degradation, starch synthesis",

author = "Tamar Azoulay-Shemer and Nikki Schwankl and Ido Rog and Menachem Moshelion and Schroeder, {Julian I.}",

note = "Funding Information: This work was funded by a grant from the National Science Foundation (grant number MCB-1616236) to JIS and was in part supported by postdoctoral fellowships from the US-Israel Binational Agricultural Research and Development Fund (BARD F1-446-11) to TA-S, by the UCSD Frontiers of Innovation Scholars Program (FISP) to TA-S and by the Israel Ministry of Aliyah and Integration. Publisher Copyright: {\textcopyright} 2018 Federation of European Biochemical Societies",

year = "2018",

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doi = "10.1002/1873-3468.13198",

language = "American English",

volume = "592",

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AU - Azoulay-Shemer, Tamar

AU - Schwankl, Nikki

AU - Rog, Ido

AU - Moshelion, Menachem

AU - Schroeder, Julian I.

N1 - Funding Information: This work was funded by a grant from the National Science Foundation (grant number MCB-1616236) to JIS and was in part supported by postdoctoral fellowships from the US-Israel Binational Agricultural Research and Development Fund (BARD F1-446-11) to TA-S, by the UCSD Frontiers of Innovation Scholars Program (FISP) to TA-S and by the Israel Ministry of Aliyah and Integration. Publisher Copyright: © 2018 Federation of European Biochemical Societies

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N2 - Starch in guard cells functions in osmoregulation during stomatal movements. Starch metabolism is controlled by the circadian clock. We investigated the role of starch metabolism in stomatal responses to CO2 under different photoperiodic conditions. Guard cell starch levels correlate with low/high [CO2] exposure. Starch biosynthesis-deficient AGPase (ADG1) mutants but, unexpectedly, not the starch degradation-deficient BAM1, BAM3, and SEX1 mutants alone, are rate-limiting for stomatal conductance responses to [CO2]-shifts. Interestingly, AGPase is rate-limiting solely under short- but not long-day conditions. These findings suggest a model of enhanced AGPase activity in guard cells under short days such that starch biosynthesis becomes rate-limiting for CO2-induced stomatal closing.

AB - Starch in guard cells functions in osmoregulation during stomatal movements. Starch metabolism is controlled by the circadian clock. We investigated the role of starch metabolism in stomatal responses to CO2 under different photoperiodic conditions. Guard cell starch levels correlate with low/high [CO2] exposure. Starch biosynthesis-deficient AGPase (ADG1) mutants but, unexpectedly, not the starch degradation-deficient BAM1, BAM3, and SEX1 mutants alone, are rate-limiting for stomatal conductance responses to [CO2]-shifts. Interestingly, AGPase is rate-limiting solely under short- but not long-day conditions. These findings suggest a model of enhanced AGPase activity in guard cells under short days such that starch biosynthesis becomes rate-limiting for CO2-induced stomatal closing.

KW - Arabidopsis

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