Statistical thermodynamics of transcription profiles in normal development and tumorigeneses in cohorts of patients

F. Remacle, R. D. Levine*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Experimental biology is providing the distribution of numerous different biological molecules inside cells and in body fluids of patients. Statistical methods of analysis have very successfully examined these rather large databases. We seek to use a thermodynamic analysis to provide a physical understanding and quantitative characterization of human cancers and other pathologies within a molecule-centered approach. The key technical development is the introduction of a Lagrangian. By imposing constraints the minimal value of the Lagrangian defines a thermodynamically stable state of the cellular system. The minimization also allows using experimental data measured at a number of different conditions to evaluate the steady-state distribution of biomolecules such as messenger RNAs. Thereby the number of effectively accessible quantum states of biomolecules is determined from the experimentally measured expression levels. With the increased resolution provided by the minimization of the Lagrangian one can differentiate between normal and diseased patients and further between disease subtypes. Each such refinement corresponds to imposing an additional constraint of biological origin. The constraints are the unbalanced ongoing biological processes in the system. MicroRNA expression level data for control and diseased lung cancer patients are analyzed as an example.

Original languageEnglish
Pages (from-to)709-726
Number of pages18
JournalEuropean Biophysics Journal
Volume44
Issue number8
DOIs
StatePublished - 20 Aug 2015

Bibliographical note

Publisher Copyright:
© 2015, European Biophysical Societies' Association.

Keywords

  • Cancer heterogeneity
  • Genomics
  • Maximal entropy
  • Minimal free energy
  • Surprisal analysis
  • Systems biology

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