TY - JOUR
T1 - Stimulation of adenine nucleotide translocation in reconstituted vesicles by phosphate and the phosphate transporter
AU - Shertzer, Howard G.
AU - Kanner, Baruch I.
AU - Banerjee, Ranajit K.
AU - Racker, Efraim
PY - 1977/4/11
Y1 - 1977/4/11
N2 - Highly purified adenine nucleotide transporter from bovine heart mitochondria was reconstituted with phospholipids to form vesicles which catalyzed atractyloside-sensitive adenine nucleotide translocation. When internal ATP was exchanged with external ADP, this reaction was enhanced by agents capable of collapsing a membrane potential, but not by inorganic phosphate. When the purified nucleotide transporter was reconstituted together with a second protein fraction, nucleotide transport was stimulated by inorganic phosphate. The stimulated rate was eliminated by mersalyl or other SH reagents. The second protein fraction could be replaced by preparations of purified phosphate transporter.
AB - Highly purified adenine nucleotide transporter from bovine heart mitochondria was reconstituted with phospholipids to form vesicles which catalyzed atractyloside-sensitive adenine nucleotide translocation. When internal ATP was exchanged with external ADP, this reaction was enhanced by agents capable of collapsing a membrane potential, but not by inorganic phosphate. When the purified nucleotide transporter was reconstituted together with a second protein fraction, nucleotide transport was stimulated by inorganic phosphate. The stimulated rate was eliminated by mersalyl or other SH reagents. The second protein fraction could be replaced by preparations of purified phosphate transporter.
UR - http://www.scopus.com/inward/record.url?scp=0017335788&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(77)91540-6
DO - 10.1016/0006-291X(77)91540-6
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C2 - 856185
AN - SCOPUS:0017335788
SN - 0006-291X
VL - 75
SP - 779
EP - 784
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -