Stimulation of human peripheral blood lymphocytes by autologous EBV-infected B cells

Jean Viallat; Erik Svedmyr; Eitan Yefenof; George Klein; Ola Weiland

doi:10.1016/S0008-8749(78)80023-9

Stimulation of human peripheral blood lymphocytes by autologous EBV-infected B cells

Jean Viallat^*, Erik Svedmyr, Eitan Yefenof, George Klein, Ola Weiland

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

EBV carrying lymphoblastoid cell lines (LCL) strongly stimulate DNA synthesis in normal autologous peripheral T lymphocytes in vitro (autologous stimulation-AS), and generate non-specifically cytotoxic T cells. The AS reaction was explored by replacing the stimulating LCLs with recently infected B cells from normal individuals. Autologous B cells infected one day earlier with EBV induced significant DNA stimulation but generated no killer cells. The ability to generate cytotoxic T cells appeared 6 to 8 days after EBV infection. Cytotoxicity was not EBV-specific. The ability to activate the alternate complement pathway appeared at approximately the same time. B-cells of convalescent infectious mononucleosis (IM) patients could generate autologous cytotoxicity already 1 day after EBV-infection. The difference between the AS reactions induced by normal and convalescent IM donors is discussed.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Cellular Immunology
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/S0008-8749(78)80023-9
State	Published - Nov 1978
Externally published	Yes

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abstract = "EBV carrying lymphoblastoid cell lines (LCL) strongly stimulate DNA synthesis in normal autologous peripheral T lymphocytes in vitro (autologous stimulation-AS), and generate non-specifically cytotoxic T cells. The AS reaction was explored by replacing the stimulating LCLs with recently infected B cells from normal individuals. Autologous B cells infected one day earlier with EBV induced significant DNA stimulation but generated no killer cells. The ability to generate cytotoxic T cells appeared 6 to 8 days after EBV infection. Cytotoxicity was not EBV-specific. The ability to activate the alternate complement pathway appeared at approximately the same time. B-cells of convalescent infectious mononucleosis (IM) patients could generate autologous cytotoxicity already 1 day after EBV-infection. The difference between the AS reactions induced by normal and convalescent IM donors is discussed.",

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AU - Weiland, Ola

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AB - EBV carrying lymphoblastoid cell lines (LCL) strongly stimulate DNA synthesis in normal autologous peripheral T lymphocytes in vitro (autologous stimulation-AS), and generate non-specifically cytotoxic T cells. The AS reaction was explored by replacing the stimulating LCLs with recently infected B cells from normal individuals. Autologous B cells infected one day earlier with EBV induced significant DNA stimulation but generated no killer cells. The ability to generate cytotoxic T cells appeared 6 to 8 days after EBV infection. Cytotoxicity was not EBV-specific. The ability to activate the alternate complement pathway appeared at approximately the same time. B-cells of convalescent infectious mononucleosis (IM) patients could generate autologous cytotoxicity already 1 day after EBV-infection. The difference between the AS reactions induced by normal and convalescent IM donors is discussed.

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Stimulation of human peripheral blood lymphocytes by autologous EBV-infected B cells

Abstract

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