Stimulation of murine cultured mast cells under anaerobic conditions: inhibition of arachidonic acid release

M. Lerner; A. Samuni; E. Razin

doi:10.1016/0165-2478(87)90118-0

Stimulation of murine cultured mast cells under anaerobic conditions: inhibition of arachidonic acid release

M. Lerner, A. Samuni, E. Razin^*

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The exocytosis of β-hexosaminidase from either IgE-antigen- or calcium ionophore A23187-stimulated murine bone-marrow-derived mast cells was not affected by oxygen-depleted conditions regardless of the absence of glucose from the medium. No detectable changes in the content of ATP were observed when the cells were triggered immunologically under anaerobic conditions in the absence of glucose in the medium. Depletion of oxygen from mast cells activated by both stimuli almost completely inhibited the specific release of arachidonic acid, which indicates that arachidonate does not play a significant role in the secretion of preformed mediators.

Original language	English
Pages (from-to)	121-124
Number of pages	4
Journal	Immunology Letters
Volume	16
Issue number	2
DOIs	https://doi.org/10.1016/0165-2478(87)90118-0
State	Published - Nov 1987

Keywords

Anaerobic conditions
Arachidonic acid
Mast cell

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10.1016/0165-2478(87)90118-0

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title = "Stimulation of murine cultured mast cells under anaerobic conditions: inhibition of arachidonic acid release",

abstract = "The exocytosis of β-hexosaminidase from either IgE-antigen- or calcium ionophore A23187-stimulated murine bone-marrow-derived mast cells was not affected by oxygen-depleted conditions regardless of the absence of glucose from the medium. No detectable changes in the content of ATP were observed when the cells were triggered immunologically under anaerobic conditions in the absence of glucose in the medium. Depletion of oxygen from mast cells activated by both stimuli almost completely inhibited the specific release of arachidonic acid, which indicates that arachidonate does not play a significant role in the secretion of preformed mediators.",

keywords = "Anaerobic conditions, Arachidonic acid, Mast cell",

author = "M. Lerner and A. Samuni and E. Razin",

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T2 - inhibition of arachidonic acid release

AU - Lerner, M.

AU - Samuni, A.

AU - Razin, E.

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Y1 - 1987/11

N2 - The exocytosis of β-hexosaminidase from either IgE-antigen- or calcium ionophore A23187-stimulated murine bone-marrow-derived mast cells was not affected by oxygen-depleted conditions regardless of the absence of glucose from the medium. No detectable changes in the content of ATP were observed when the cells were triggered immunologically under anaerobic conditions in the absence of glucose in the medium. Depletion of oxygen from mast cells activated by both stimuli almost completely inhibited the specific release of arachidonic acid, which indicates that arachidonate does not play a significant role in the secretion of preformed mediators.

AB - The exocytosis of β-hexosaminidase from either IgE-antigen- or calcium ionophore A23187-stimulated murine bone-marrow-derived mast cells was not affected by oxygen-depleted conditions regardless of the absence of glucose from the medium. No detectable changes in the content of ATP were observed when the cells were triggered immunologically under anaerobic conditions in the absence of glucose in the medium. Depletion of oxygen from mast cells activated by both stimuli almost completely inhibited the specific release of arachidonic acid, which indicates that arachidonate does not play a significant role in the secretion of preformed mediators.

KW - Anaerobic conditions

KW - Arachidonic acid

KW - Mast cell

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Stimulation of murine cultured mast cells under anaerobic conditions: inhibition of arachidonic acid release

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