Stirring Peptide Synthesis to a New Level of Efficiency

Johnny N. Naoum, Israel Alshanski, Guy Mayer, Poriah Strauss, Mattan Hurevich*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

8 Scopus citations

Abstract

Accelerating solid-phase synthesis is crucial for accessing a large number of peptides in a short time. Since standard peptide synthesis is usually done under poor diffusion conditions with slow or no mixing of the solid support, acceleration of the process is achieved by applying a large excess of reagents. In this work, overhead stirring and heating were combined to provide accelerated solid-phase peptide synthesis without using an excess of reagent. A new setup that allows both heating and fast stirring was designed specifically for research laboratory-scale peptide synthesis. By increasing the diffusion of both reagents and beads in a narrow dimension reactor, solid-phase reactions were done in seconds and medium-size peptides were synthesized in minutes.

Original languageAmerican English
Pages (from-to)129-136
Number of pages8
JournalOrganic Process Research and Development
Volume26
Issue number1
DOIs
StatePublished - 21 Jan 2022

Bibliographical note

Publisher Copyright:
© 2022 American Chemical Society

Keywords

  • Fick’s second law
  • diffusion
  • peptides
  • reactor design
  • solid-phase synthesis

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