TY - JOUR
T1 - Strain-dependent activation of the mouse immune response is correlated with Porphyromonas gingivalis-induced experimental periodontitis
AU - Wilensky, Asaf
AU - Polak, David
AU - Awawdi, Suhair
AU - Halabi, Amal
AU - Shapira, Lior
AU - Houri-Haddad, Yael
PY - 2009/11
Y1 - 2009/11
N2 - Aims: To evaluate the effect of oral infection with three Porphyromonas gingivalis strains on alveolar bone loss (ABL) and its correlation with the mouse immune response. Materials and Methods: Mice were orally infected with P. gingivalis strains 381, 33277 and 53977. After 42 days, maxillae were analysed for ABL using micro-computed tomography and the serum for anti-P.gingivalis IgG1 and IgG2a levels. The cytokine response to P. gingivalis was tested using the subcutaneous chamber model. Results: The P. gingivalis 53977-infected group showed the highest ABL, which was significantly different from all other groups (p<0.001). In addition, the humoral response to P. gingivalis 53977 was significantly lower than the response to P. gingivalis 381 and 33277 (p≤0.01). The IgG2a/IgG1 ratio was higher in the P. gingivalis 33277-infected group (1.6) compared with the P. gingivalis 381-infected group (0.51). Four days post-infection, interleukin (IL)-1β levels remained significantly higher in the P. gingivalis 53977-infected group only (1198.2±260.0, p<0.05), while IL-4 levels remained significantly higher in the P. gingivalis 381-infected group (265.8±131.6, p<0.05). Conclusions: The high levels of ABL induced by P. gingivalis 53977 were inversely correlated with the humoral response to this bacterium. In addition, ABL was correlated with an elevated pro-inflammatory response.
AB - Aims: To evaluate the effect of oral infection with three Porphyromonas gingivalis strains on alveolar bone loss (ABL) and its correlation with the mouse immune response. Materials and Methods: Mice were orally infected with P. gingivalis strains 381, 33277 and 53977. After 42 days, maxillae were analysed for ABL using micro-computed tomography and the serum for anti-P.gingivalis IgG1 and IgG2a levels. The cytokine response to P. gingivalis was tested using the subcutaneous chamber model. Results: The P. gingivalis 53977-infected group showed the highest ABL, which was significantly different from all other groups (p<0.001). In addition, the humoral response to P. gingivalis 53977 was significantly lower than the response to P. gingivalis 381 and 33277 (p≤0.01). The IgG2a/IgG1 ratio was higher in the P. gingivalis 33277-infected group (1.6) compared with the P. gingivalis 381-infected group (0.51). Four days post-infection, interleukin (IL)-1β levels remained significantly higher in the P. gingivalis 53977-infected group only (1198.2±260.0, p<0.05), while IL-4 levels remained significantly higher in the P. gingivalis 381-infected group (265.8±131.6, p<0.05). Conclusions: The high levels of ABL induced by P. gingivalis 53977 were inversely correlated with the humoral response to this bacterium. In addition, ABL was correlated with an elevated pro-inflammatory response.
KW - Alveolar bone loss
KW - Experimental periodontitis
KW - Interleukin-1β
KW - Interleukin-4
KW - Porphyromonas gingivalis
UR - http://www.scopus.com/inward/record.url?scp=70350179966&partnerID=8YFLogxK
U2 - 10.1111/j.1600-051X.2009.01464.x
DO - 10.1111/j.1600-051X.2009.01464.x
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C2 - 19735468
AN - SCOPUS:70350179966
SN - 0303-6979
VL - 36
SP - 915
EP - 921
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
IS - 11
ER -