Strand-specific RNA sequencing reveals extensive regulated long antisense transcripts that are conserved across yeast species

Moran Yassour, Jenna Pfiffner, Joshua Z. Levin, Xian Adiconis, Andreas Gnirke, Chad Nusbaum, Dawn Anne Thompson*, Nir Friedman, Aviv Regev

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

Background: Recent studies in budding yeast have shown that antisense transcription occurs at many loci. However, the functional role of antisense transcripts has been demonstrated only in a few cases and it has been suggested that most antisense transcripts may result from promiscuous bi-directional transcription in a dense genome.Results: Here, we use strand-specific RNA sequencing to study anti-sense transcription in Saccharomyces cerevisiae. We detect 1,103 putative antisense transcripts expressed in mid-log phase growth, ranging from 39 short transcripts covering only the 3' UTR of sense genes to 145 long transcripts covering the entire sense open reading frame. Many of these antisense transcripts overlap sense genes that are repressed in mid-log phase and are important in stationary phase, stress response, or meiosis. We validate the differential regulation of 67 antisense transcripts and their sense targets in relevant conditions, including nutrient limitation and environmental stresses. Moreover, we show that several antisense transcripts and, in some cases, their differential expression have been conserved across five species of yeast spanning 150 million years of evolution. Divergence in the regulation of antisense transcripts to two respiratory genes coincides with the evolution of respiro-fermentation.Conclusions: Our work provides support for a global and conserved role for antisense transcription in yeast gene regulation.

Original languageAmerican English
Article numberR87
JournalGenome Biology
Volume11
Issue number8
DOIs
StatePublished - 26 Aug 2010

Bibliographical note

Funding Information:
MY was supported by the Canadian Friends of the Hebrew University. The work was supported by the Howard Hughes Medical Institute, the Human Frontiers Science Program, a Career Award at the Scientific Interface from the Burroughs Welcome Fund, an NIH PIONEER award, the Broad Institute, and a Sloan Fellowship (AR), the National Human Genome Research Institute (NHGRI) (CN), and by a US-Israel Bi-national Science Foundation award (NF and MY).

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