TY - JOUR
T1 - Streptococcus agalactiae and Streptococcus difficile 16S-23S intergenic rDNA
T2 - Genetic homogeneity and species-specific PCR
AU - Berridge, Brian R.
AU - Bercovier, Herve
AU - Frelier, Paul F.
PY - 2001/1/26
Y1 - 2001/1/26
N2 - Streptococcus difficile is a non-hemolytic Gram-positive bacterial coccus that causes septicemia and meningoencephalitis in farmed tilapia (Oreochromis sp.) and rainbow trout (Oncorhynchus mykiss). Recent studies have demonstrated S. difficile to be a group B, type Ib streptococcus with a whole cell protein electrophoretic profile indistinguishable from S. agalactiae and a biochemical profile similar to that observed for other group B, type Ib streptococci isolated from fish and frogs. The aim of this study was to expand on these findings by comparative nucleic acid sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacers of S. difficile and S. agalactiae. The 97.7% sequence homology identified in these studies supports the taxonomic relationship of these two organisms. The sequence data generated were also used to construct a pair of species-specific PCR primers for use in molecular detection and identification schemes.
AB - Streptococcus difficile is a non-hemolytic Gram-positive bacterial coccus that causes septicemia and meningoencephalitis in farmed tilapia (Oreochromis sp.) and rainbow trout (Oncorhynchus mykiss). Recent studies have demonstrated S. difficile to be a group B, type Ib streptococcus with a whole cell protein electrophoretic profile indistinguishable from S. agalactiae and a biochemical profile similar to that observed for other group B, type Ib streptococci isolated from fish and frogs. The aim of this study was to expand on these findings by comparative nucleic acid sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacers of S. difficile and S. agalactiae. The 97.7% sequence homology identified in these studies supports the taxonomic relationship of these two organisms. The sequence data generated were also used to construct a pair of species-specific PCR primers for use in molecular detection and identification schemes.
KW - 16S-23S
KW - Streptococcus agalactiae
KW - Streptococcus difficile
KW - rDNA
UR - http://www.scopus.com/inward/record.url?scp=0035951333&partnerID=8YFLogxK
U2 - 10.1016/S0378-1135(00)00285-6
DO - 10.1016/S0378-1135(00)00285-6
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C2 - 11163706
AN - SCOPUS:0035951333
SN - 0378-1135
VL - 78
SP - 165
EP - 173
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 2
ER -
