Streptococcus mutans fructosyltransferase interactions with glucans

Ramona Rozen, Doron Steinberg, Gilad Bachrach*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Streptococcus mutans utilizes sucrose to synthesize glucans by glucosyltransferase and fructans by fructosyltransferase (FTF). Antibodies raised against a recombinant FTF were used to study S. mutans FTF secretion. Low amounts of cell-free FTF were found in culture of S. mutans grown with sucrose, while an increase in bacteria displaying cell surface FTF was detected. FTF added to S. mutans cultures was adsorbed to bacteria grown with sucrose but not to bacteria grown with glucose or fructose or to a gtf inactivated mutant grown with sucrose. Recombinant FTF was found to have high affinity for glucans suggesting that fructans and glucans are an integral part of the polysaccharide matrix of oral biofilms.

Original languageAmerican English
Pages (from-to)39-43
Number of pages5
JournalFEMS Microbiology Letters
Volume232
Issue number1
DOIs
StatePublished - 12 Mar 2004
Externally publishedYes

Keywords

  • Fructosyltransferase
  • Glucan binding
  • Protein expression
  • Streptococcus mutans

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