Structural and functional alterations of cyanobacterial phycobilisomes induced by high-light stress

Eyal Tamary, Vladimir Kiss, Reinat Nevo, Zach Adam, Gábor Bernát, Sascha Rexroth, Matthias Rögner, Ziv Reich*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

Exposure of cyanobacterial or red algal cells to high light has been proposed to lead to excitonic decoupling of the phycobilisome antennae (PBSs) from the reaction centers. Here we show that excitonic decoupling of PBSs of Synechocystis sp. PCC 6803 is induced by strong light at wavelengths that excite either phycobilin or chlorophyll pigments. We further show that decoupling is generally followed by disassembly of the antenna complexes and/or their detachment from the thylakoid membrane. Based on a previously proposed mechanism, we suggest that local heat transients generated in the PBSs by non-radiative energy dissipation lead to alterations in thermo-labile elements, likely in certain rod and core linker polypeptides. These alterations disrupt the transfer of excitation energy within and from the PBSs and destabilize the antenna complexes and/or promote their dissociation from the reaction centers and from the thylakoid membranes. Possible implications of the aforementioned alterations to adaptation of cyanobacteria to light and other environmental stresses are discussed.

Original languageAmerican English
Pages (from-to)319-327
Number of pages9
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1817
Issue number2
DOIs
StatePublished - Feb 2012

Bibliographical note

Funding Information:
We thank Vlad Brumfeld (Weizmann Institute of Science) for assistance with the spectroscopic measurements. We also thank Dana Charuvi, Ruti Kapon, and Onie Tsabari (Weizmann Institute of Science) for helpful comments and suggestions. This work was supported by grants from the Israel Science Foundation ( No. 1005/07 , Z.R.), the German Research Foundation (DFG, SFB 480 , project C1, M.R.), the German Federal Ministry of Education and Research (BMBF, project Bio-H 2 , G.B. and M.R.), and the EU project Solar-H 2 (M.R.).

Keywords

  • Cyanobacteria
  • Energetic decoupling
  • Energy transfer
  • FRAP
  • Photoprotection
  • Phycobilisomes

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