Structural basis for enzyme bifunctionality – the case of Gan1D from Geobacillus stearothermophilus

Keyphrases

• Phosphate 100%
• Bifunctionality 100%
• β-Galactosidase (β-Gal) 80%
• Glucose-6-phosphatase (G6Pase) 60%
• Galactose 60%
• Glucosidase Activity 60%
• Glycoside Hydrolase 40%
• Cellobiose 40%
• Glucosidase 40%
• Active Sites 20%
• Small-angle X-ray Scattering 20%
• Organic Molecules 20%
• Quaternary Structure 20%
• Structural Information 20%
• Hydrolases 20%
• Acid-base 20%
• Gene Cluster 20%
• Enzyme Substrates 20%
• Phosphate Binding Site 20%
• Binding Mode 20%
• Binding Interaction 20%
• Galactose Utilization 20%
• Gel Filtration 20%
• Catalytic mutant 20%
• Enzyme Product 20%
• Degradation Products 20%
• Dual Activity 20%
• Protein Data Bank 20%
• Tertiary Structure 20%
• Glycosidic Bond 20%
• Specific Enzymes 20%
• Aglycone 20%
• Binding Residues 20%
• Enzyme Specificity 20%
• Dimer Assembly 20%
• 3D Crystal Structure 20%
• Glycone 20%
• Bifunctional Activity 20%
• Structure-function Relationship 20%
• Structural Bioinformatics 20%
• Collaboratory 20%
• Specific mutation 20%
• Atomic Coordinates 20%
• TIM-barrel Fold 20%
• Non-reducing 20%

Biochemistry, Genetics and Molecular Biology

• Enzyme 100%
• Glucosidase 83%
• Wild Type 66%
• Galactosidase 66%
• Galactose 50%
• Glucose 6-Phosphate 50%
• Active Site 33%
• Glycoside Hydrolase 33%
• Cellobiose 33%
• X Ray 16%
• Crystal Structure 16%
• Binding Site 16%
• Enzymatic Hydrolysis 16%
• Protein Quaternary Structure 16%
• Enzyme Specificity 16%
• Gene Cluster 16%
• Enzyme Substrate 16%
• Galactan 16%
• Gel Filtration 16%
• Protein Data Bank 16%
• Tertiary Structure 16%
• Glycosidic Bond 16%
• TIM Barrel 16%
• Aglycone 16%