Structural properties and release of insulin-loaded reverse hexagonal (HII) liquid crystalline mesophase

Tehila Mishraki-Berkowitz, Abraham Aserin, Nissim Garti*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Insulin loading into the HII mesophases was examined as a function of its concentration, with addition of glycerol as a cosolvent and with addition of phosphatidylcholine (PC) as a structural stabilizer. The structural properties, the molecular interactions, the viscoelastic properties, and the dynamic behavior were investigated by SAXS, ATR-FTIR, and rheological measurements. Insulin release was then monitored and analyzed. Insulin incorporation into the HII systems shrank the cylinders as it competed with the lipids in water-bonding. Insulin interrupted the interface while increasing τmax and creating a more solid-like response. Upon addition of PC, cooperative flow behavior was detected, which is probably the reason for increase in insulin cumulative release from 28% to 52% after 300 min. In the presence of glycerol, the system was less cooperative but insulin was more compactly folded, resulting in a slight improvement in insulin release (up to 6%). Addition of both PC and glycerol caused the maximum release (55%). The addition of additives into the HII system demonstrates how structural modifications can improve insulin release, and influence future design of encapsulated drug delivery systems.

Original languageEnglish
Pages (from-to)184-193
Number of pages10
JournalJournal of Colloid and Interface Science
Volume486
DOIs
StatePublished - 15 Jan 2017

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.

Keywords

  • ATR-FTIR
  • Glycerol
  • Glycerol monooleate
  • Insulin
  • Phosphatidylcholine
  • Release
  • Reverse hexagonal mesophase
  • Rheology
  • SAXS

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