TY - JOUR
T1 - Structural properties and release of insulin-loaded reverse hexagonal (HII) liquid crystalline mesophase
AU - Mishraki-Berkowitz, Tehila
AU - Aserin, Abraham
AU - Garti, Nissim
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2017/1/15
Y1 - 2017/1/15
N2 - Insulin loading into the HII mesophases was examined as a function of its concentration, with addition of glycerol as a cosolvent and with addition of phosphatidylcholine (PC) as a structural stabilizer. The structural properties, the molecular interactions, the viscoelastic properties, and the dynamic behavior were investigated by SAXS, ATR-FTIR, and rheological measurements. Insulin release was then monitored and analyzed. Insulin incorporation into the HII systems shrank the cylinders as it competed with the lipids in water-bonding. Insulin interrupted the interface while increasing τmax and creating a more solid-like response. Upon addition of PC, cooperative flow behavior was detected, which is probably the reason for increase in insulin cumulative release from 28% to 52% after 300 min. In the presence of glycerol, the system was less cooperative but insulin was more compactly folded, resulting in a slight improvement in insulin release (up to 6%). Addition of both PC and glycerol caused the maximum release (55%). The addition of additives into the HII system demonstrates how structural modifications can improve insulin release, and influence future design of encapsulated drug delivery systems.
AB - Insulin loading into the HII mesophases was examined as a function of its concentration, with addition of glycerol as a cosolvent and with addition of phosphatidylcholine (PC) as a structural stabilizer. The structural properties, the molecular interactions, the viscoelastic properties, and the dynamic behavior were investigated by SAXS, ATR-FTIR, and rheological measurements. Insulin release was then monitored and analyzed. Insulin incorporation into the HII systems shrank the cylinders as it competed with the lipids in water-bonding. Insulin interrupted the interface while increasing τmax and creating a more solid-like response. Upon addition of PC, cooperative flow behavior was detected, which is probably the reason for increase in insulin cumulative release from 28% to 52% after 300 min. In the presence of glycerol, the system was less cooperative but insulin was more compactly folded, resulting in a slight improvement in insulin release (up to 6%). Addition of both PC and glycerol caused the maximum release (55%). The addition of additives into the HII system demonstrates how structural modifications can improve insulin release, and influence future design of encapsulated drug delivery systems.
KW - ATR-FTIR
KW - Glycerol
KW - Glycerol monooleate
KW - Insulin
KW - Phosphatidylcholine
KW - Release
KW - Reverse hexagonal mesophase
KW - Rheology
KW - SAXS
UR - http://www.scopus.com/inward/record.url?scp=84988958291&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2016.09.074
DO - 10.1016/j.jcis.2016.09.074
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C2 - 27701016
AN - SCOPUS:84988958291
SN - 0021-9797
VL - 486
SP - 184
EP - 193
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
ER -